Research Journal of Pharmacology and Pharmacodynamics

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Nephroprotective Activity of Vigna Mungo (Linn.) Hepper on Gentamicin-Induced Renal Damage in Albino Rats


Affiliations
1 Department of Pharmacology, H. K. E. Society's College of Pharmacy, Sedam Road, Gulbarga - 585105, Karnataka, India
2 Department of Pharmacology H. K. E. Society's College of Pharmacy, Sedam Road, Gulbarga - 585105, Karnataka, India
     

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The present study was undertaken to investigate the preliminary phytochemical studies and nephroprotective activity of aqueous and methanolic extracts of seeds of Vigna mungo in Gentamicin-induced nephrotoxicity in rats. The seed powder of Vigna mungo was successively extracted with methanol and water. Preliminary phytochemical tests were done, the extracts showed the presence of amino acids, alkaloids, ascorbic acid, carbohydrates, flavonoids, glycosides, proteins, phytic acid, total phenolic compounds, reducing sugars, saponins and tannins. The protective activity of the extracts was justified by physical (decrease in body weight) and biochemical (increased blood urea nitrogen, serum creatinine, serum uric acid and decreased serum total protein) changes induced by gentamicin in kidney parameters. When compared to normal control group. The groups treated with both the extracts had significantly prevented the above changes produced by gentamicin in rats. In general both the extracts possessed protective activity though methanolic extract was found to exhibit greater protection.

Keywords

Gentamicin, Nephroprotective Activity, Vigna Mungo, Phytochemistry.
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  • Shah, S.V. (1984). Effect of enzymatically generated reactive oxygen metabolites on the cyclic nucleotide content in isolated glomeruli. J Clin Invest 74:393-401.

  • Shah, S.V., Barcos, W.H., Basci, A. (1987). Degradation of human glomerular basement membrane by stimulated neutrophils. Activitation of a metalloproteinase by reactive oxygen metabolite. J Clin Invest 79:25-31.

  • Homes, H.D., Weinberg, J.M. (1986). Toxic nephropathies. In: Kidney. Brenner, B.M., Rector, F.C. Jr. (eds). Philadelphia, Saunders Co., 1491-533.

  • Ramsammy, L.S., Josephowitz, C., Ling, K.Y., Lane, B.P., Kaloyanides, G.J. (1986). Effects of diphenyl-phenylenediamine on gentamicin-induced lipid peroxidation and toxicity in rats. J Pharmacol Exp Ther. 238:83-8.

  • Walker, P.D., Shah, S.V. (1987). Gentamicin enhances production of hydrogen peroxide by renal cortical mitochondria. J Physiol. 253:495-9.

  • Walker, P.D., Shah, S.V. (1988). Evidence suggesting a role for hydroxyl radical in gentamicin-induced acute renal failure in rats. J Clin Invest. 81:334-41.

  • Kirtikar, K.R. and Basu, B.D. (1993). Indian Medicinal plants. International Book Distributors: Dehradun. 1:795-796.

  • Rehan, A.K., Johnson, K.J., Kunkel, R.G., Wiggins, R.C. (1985). Role of oxygen radicals in phorbol myrstate acetate induced glomerular injury. Kidney Inter. 27:503-11.

  • Manisha chikane, R., Dilip parwate, V., Vishwas ingle, N., et al. (2011). In vitro Antioxidant effect of seed coat extracts of Vigna mungo Journal of pharmacy Research. 4(3):656-657.

  • Khandelwal, K.R. (2000). Practical pharmacognosy technique and experiments 9th ed. Pune: Nirali Prakashan. 149-55.

  • Solanki, Y.B. and Jain, S.M. (2011). Hepatoprotective activity of Clitoria ternatea and Vigna mungo against Acetaminophen and CCl4-induced hepatotoxicity in rats Journal of pharmacol and Toxi. 6(1):30-48.

  • Vijay kumar, K., Naidu, M.U.R., Anwar shifow, A., Ratnakar, K.S. (2000). Probucol protects against gentamicin-induced nephrotoxicity in rats. Ind J Pharmacol. 32:108-113.

  • Sakhae K, Adams-Huet B, Moe OW and Pak CC. Pathophysiologic basis for normouricosuric uric acid nephrolithiasis, Kidney Int 2002; 62(3):971-979.

  • Abdel-Gayoum, A.A., Ali, B.H., Abdel Razig, K.M., Bashir, A.A., Ghywarsua, K. (1994). Effect of gentamicin-induced nephrotoxicity on some carbohydrate metabolism pathways in the rat renal cortex. Arch Toxicol. 68:643-7.

  • Shyamladevi, C.S. and Devipriya, S. (1999). Indian J. Pharmacol. 31:422-426.

  • Shelke, T.T., Kothari, R. (2009). Nephro-protective activity of ethanolic extract of dried fruits of Peddlium murex Linn. J. Cell and Tissue Research 9(1):1687-1690.

  • Ramsammy, L.S., Josephowitz, C., Ling, K.Y., Lane, B.P., Kaloyanides, G.J. (1987). Failure of inhibition of lipid peroxidation by vitamin E to protect against gentamicin-induced nephrotoxicity in the rat. Biochem Pharmacol. 36:2125-32.

  • Yizhong, C., Luo, Q., Mei, S., Corke, H. (2004). Antioxidant activity and Phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 74:2157- 2184.

  • Halvorsen, B.L., Calrsen, M.H., Philips, K.M., Bohn, S.K., et al. (2006). Content of redox-active compounds (i.e. antioxidants) in foods consumed in the United States. Am J Clin. Nutr. 84:95-135.

  • Chowdhurry, A.K.A., Jahioullah, I.J., Tabukder, S.A., Khan, A.K.A. (1987). Diuretic activities of saponins of Vigna spp. J Bangladesh Acad Sci. 11:75


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  • Nephroprotective Activity of Vigna Mungo (Linn.) Hepper on Gentamicin-Induced Renal Damage in Albino Rats

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Authors

M. Nitin
Department of Pharmacology, H. K. E. Society's College of Pharmacy, Sedam Road, Gulbarga - 585105, Karnataka, India
S. Ifthekar
Department of Pharmacology H. K. E. Society's College of Pharmacy, Sedam Road, Gulbarga - 585105, Karnataka, India

Abstract


The present study was undertaken to investigate the preliminary phytochemical studies and nephroprotective activity of aqueous and methanolic extracts of seeds of Vigna mungo in Gentamicin-induced nephrotoxicity in rats. The seed powder of Vigna mungo was successively extracted with methanol and water. Preliminary phytochemical tests were done, the extracts showed the presence of amino acids, alkaloids, ascorbic acid, carbohydrates, flavonoids, glycosides, proteins, phytic acid, total phenolic compounds, reducing sugars, saponins and tannins. The protective activity of the extracts was justified by physical (decrease in body weight) and biochemical (increased blood urea nitrogen, serum creatinine, serum uric acid and decreased serum total protein) changes induced by gentamicin in kidney parameters. When compared to normal control group. The groups treated with both the extracts had significantly prevented the above changes produced by gentamicin in rats. In general both the extracts possessed protective activity though methanolic extract was found to exhibit greater protection.

Keywords


Gentamicin, Nephroprotective Activity, Vigna Mungo, Phytochemistry.

References