Research Journal of Pharmacology and Pharmacodynamics

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Ameliorative Role of Threshold Level of Bilirubin in Newborn Jaundice and Efficacy of Noni Fruit Extract on Phenyl Hydrazine Induced Jaundice Rat


Affiliations
1 Department of Bio-Chemistry, S.R.M. Arts and Science College, Kattankulathur-603203, India
2 Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600 025, India
     

   Subscribe/Renew Journal


The aims of the present work were to elucidate the role of bilirubin as a free radical scavenger in unconjugated hyperbilirubinemic newborns. Bilirubin above an optimum level is toxic to human systems and is extcreted in urinary and through gastrointestinal tract. It is observed that serum bilirubin up to 85μ mole/l (5mg/dl) has a free radical scavenger activity and exceeding 200μmole/l (12mg/dl) is highly pro-oxidant. Phototherapy is the accepted therapeutic management of jaundiced newborns and has been shown to augment the oxidative stress. The ethanolic extract of Noni fruit administered orally at a dose of 3mg/ml is found to diminish the oxidative stress in erythrocytes of phenylhydrazine–induced unconjugated hyperbilirubinemic jaundiced rats, treated with phototherapy, which ultimately reduces the bilirubin level without inducing additional damages.

Keywords

Bilirubin, Free Radical Scavenger, Pro-Oxidant, Phototherapy, Jaundice, Oxidative Stress, Noni.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Text book of Pediatrics (Behrman R E, Vanghen V C, Nelson W E and Saunders W B, eds), Philadelphia, USA, (1992) 14th edn: 476

  • Murray R K Harper's Illustrated Biochemistry, McGraw Hill,USA (2003) 26th edn, 270-285

  • Kaplan M, Muraca M, Hammerman C, Rubaltelli F F, Vilei MT, Vreman H J and Stevenson D K Pediatrics (2002) 110, e47 4. Mazumder S, Sarkar U and Sengupta D. Indian J Exp Biol(1995) 33: 303-305

  • Mazumder S, Sarkar U and Sengupta D Indian J Exp Biol (2000) 38 :91-94

  • Wennberg R P, Ahlfors C E, Bhutani V K, Johnson L H and Shapiro S M Pediatrics(2006) 117:474-485

  • Kiran Dahiya, A.D.Tiwari, Vijay Shankar, Simmi Kharb, Rakesh Dhankhar,. Indian J. Cli. Biochem. (2006):21(1) 157-160

  • N Nag, S Halder, R Chaudhuri, S Adhikary and S Mazumder, Indian Journal of Biochemistry and Biophysics( 2009) 46:73-78.

  • Bader D, Kugelman A, Blum D E, Riskin A and Tirosh E. IMAJ (2006) 8: 12-16

  • Greenberg D A Proc Natl Acad Sci (2002) 99: 15837-15839

  • Drabkin DL, Austin JH J. Biol. Chem. (1932) 98:719-733

  • Kumar, R., Seth, R.K., Sekhon, M.S. and Bhargava, J.S., Burn. (1995) 21: 96-97.

  • Ellman, G.L.. Arch. Biochem. Biophys. (1959). 82: 70-77.

  • Beutler, E., Duron, O. and Kelly, B.M. J. Lab. Clin. Med.(1963). 61: 882-888.

  • Mc Cormick, D.B. and Greene, H.L Vitamins, In: Teitz Textbook of Clinical Chemistry Eds. Burtis,CA and Ashwood, 999-1028.

  • Doumas, B.T. and Biggs, H.G.). Chem. (1972) 7:175-188.

  • Misra, HP. and Fridovich, I. J. Biol. Chem. (1972) 247: 3170-3175.

  • Sun J, Schulman S G and Perrin J H Anal Chem Acta (1997) 338: 1-2

  • Valentine J S and Curtis AB J Am Chem Soc (1975) 97: 224-226

  • Larson E A, Evans G T and Watson CJ J Lab Clin Med (1947) 32: 481-488

  • Varley H, Gowenlock A H and Bell M Practical Clinical Biochemistry, 5th edn, W H Med Book Ltd. London (1984) 1024-1027

  • Malloy H T and Evelyn K A J. Biol Chem. (1937) 119: 481-90

  • Cascone O, Frydman R B, Ferrara P, Tomaro M L and Rosenfeld J Eur J Biochem (1989) 179, 123-130

  • Lohr C W and Waller H D In: Methods in enzymology (Bergmeyer H ed), Academic Press, New York (1974),636

  • Yamada K and Shimazono W J Biochem (1962) 51: 242-245

  • Horecker B L and Symrniotis P Z In: Methods in enzymology (Colowick S P, Kaplan N O, eds), Academic Press, New York(1955) Vol. 1: 371

  • Mellors A and Tappel A L J Biol Chem (1966) 241: 4353-4356

  • Liu Y, Shi J and Langrish T A G Chem Eng J (2006) 120: 203-209

  • Huang W, Zhang J, Chua S S, Qatanani M, Han Y, Granata R and Moore D D Proc Natl Acad Sci (USA) (2003) 100: 4156-4161

  • Gallucci M T, Lubrano R, Meloni C, Morosetti M, Manca di Villahermosa S, Scoppi P, Palombo G, Castello M A and Casciani C U Clini Nephrol(1999) 52:239-245

  • Esterbaur H, Schauer R J and Zollner H Free Radic Biol Med (1991) 11: 81-128

  • Yoritaka A, Hattori N, Uchida K, Tanaka M, Stadtman E R and Mizuno Y Proc Natl Acad Sci(1996) 93:2696-2701

  • Lowry O H and Lopez J A J Biol Chem (1946) 162:421-428

  • Jain S K Free Radic Biol Med (1998) 24:197-201

  • Ostrea, E.M. Jr., Cepeda, E.E., Fleury, C.A. and Balun, J.E Acta. Pediatr. (1985) 74:378-381

  • BracciR,TalluriB and BerniS. Acta. Paediatr. Scan(1988) 77 (3): 349-356.

  • Turgut, M., Basaran, O., Cekmen, M., Karatas, F., Kurt, A. and Aygun, A.D. J. Paediatr. Child Health. (2004). 40 (11): 633-637.

  • Mahadeva Rao.US and Subramanian S. J. Med. Chem. Res (2009) 18:433-446


Abstract Views: 303

PDF Views: 0




  • Ameliorative Role of Threshold Level of Bilirubin in Newborn Jaundice and Efficacy of Noni Fruit Extract on Phenyl Hydrazine Induced Jaundice Rat

Abstract Views: 303  |  PDF Views: 0

Authors

U. S. Mahadeva Rao
Department of Bio-Chemistry, S.R.M. Arts and Science College, Kattankulathur-603203, India
B. Arirudran
Department of Bio-Chemistry, S.R.M. Arts and Science College, Kattankulathur-603203, India
S. Subramanian
Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600 025, India

Abstract


The aims of the present work were to elucidate the role of bilirubin as a free radical scavenger in unconjugated hyperbilirubinemic newborns. Bilirubin above an optimum level is toxic to human systems and is extcreted in urinary and through gastrointestinal tract. It is observed that serum bilirubin up to 85μ mole/l (5mg/dl) has a free radical scavenger activity and exceeding 200μmole/l (12mg/dl) is highly pro-oxidant. Phototherapy is the accepted therapeutic management of jaundiced newborns and has been shown to augment the oxidative stress. The ethanolic extract of Noni fruit administered orally at a dose of 3mg/ml is found to diminish the oxidative stress in erythrocytes of phenylhydrazine–induced unconjugated hyperbilirubinemic jaundiced rats, treated with phototherapy, which ultimately reduces the bilirubin level without inducing additional damages.

Keywords


Bilirubin, Free Radical Scavenger, Pro-Oxidant, Phototherapy, Jaundice, Oxidative Stress, Noni.

References