Research Journal of Pharmacology and Pharmacodynamics

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The Frequency of the most Potentially Toxigenic Fungi in Broiler Feeds in Kermanshah Province, West of Iran


Affiliations
1 Department of Pathobiology, Razi University, Kermanshah, Iran, Islamic Republic of
2 Department of Microbiology, Faculty of Science, Islamic Azad University, Karaj Branch, Karaj, Iran, Islamic Republic of
3 Department of Medical and Veterinary Mycology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran, Islamic Republic of
     

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The objectives of this study were to investigate the occurrence and identification of the most potentially toxigenic fungi in broiler feeds in Kermanshah province, west of Iran. From April 2008 to March 2009, a total of 100 broiler feed samples were aseptically collected from different broiler farms located in the province. Samples were transported to the laboratory, homogenized, quartered to obtain a one kg laboratory sample and were stored at 4°C for fungal analyses. Ten grams of each feed sample were homogenized in 90 ml sterile physiological saline for 30 minutes to obtain a concentration of 10-1 (dilution 1). Then this mixture was serially diluted to 10-2 (dilution 2), 10-3 (dilution 3) and 10-4 (dilution 4). From each dilution, 25μL of the mixture was deeply inoculated on dichloran rose-bengal-chloranphenicol agar(DRBC) and incubated at 25°C for 5 to 15 days. During the incubation period, gross and microscopic features of fungal colonies were studied. Statistical analyses of the data were done using SPSS software (Version 16). Aspergillus Spp. were the most prevalent; they could be isolated from 92 samples (51.1 %) in dilution 1, 76 samples (59.3%) in dilution 2, 56 samples (66.6%)m' dilution 3 and 40 samples (83%) in dilution 4. Fusarium spp. Were isolated from 52 samples (28.8%) in dilution 1, 28 samples (21.8%) in dilution 2, 20 samples (23.8%) in dilution 3 and 8 samples (16%) in dilution 4. Penicillium spp. Could be isolated from 36 samples (20%) in dilution 1, 24 samples (18.7%) in dilution 2, 8 samples (9%) in dilution 3 and 0 samples (0%)) in dilution 4. The results of this study indicate the high rate of contamination of broiler feeds to common toxigenic fungi in western parts of Iran.

Keywords

Toxigenic Fungi,Mycotoxins, Broiler Feed, Kermanshah Province.
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  • The Frequency of the most Potentially Toxigenic Fungi in Broiler Feeds in Kermanshah Province, West of Iran

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Authors

Yousef Azarakhsh
Department of Pathobiology, Razi University, Kermanshah, Iran, Islamic Republic of
Azar Sabokbar
Department of Microbiology, Faculty of Science, Islamic Azad University, Karaj Branch, Karaj, Iran, Islamic Republic of
Mansour Bayat
Department of Medical and Veterinary Mycology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran, Islamic Republic of

Abstract


The objectives of this study were to investigate the occurrence and identification of the most potentially toxigenic fungi in broiler feeds in Kermanshah province, west of Iran. From April 2008 to March 2009, a total of 100 broiler feed samples were aseptically collected from different broiler farms located in the province. Samples were transported to the laboratory, homogenized, quartered to obtain a one kg laboratory sample and were stored at 4°C for fungal analyses. Ten grams of each feed sample were homogenized in 90 ml sterile physiological saline for 30 minutes to obtain a concentration of 10-1 (dilution 1). Then this mixture was serially diluted to 10-2 (dilution 2), 10-3 (dilution 3) and 10-4 (dilution 4). From each dilution, 25μL of the mixture was deeply inoculated on dichloran rose-bengal-chloranphenicol agar(DRBC) and incubated at 25°C for 5 to 15 days. During the incubation period, gross and microscopic features of fungal colonies were studied. Statistical analyses of the data were done using SPSS software (Version 16). Aspergillus Spp. were the most prevalent; they could be isolated from 92 samples (51.1 %) in dilution 1, 76 samples (59.3%) in dilution 2, 56 samples (66.6%)m' dilution 3 and 40 samples (83%) in dilution 4. Fusarium spp. Were isolated from 52 samples (28.8%) in dilution 1, 28 samples (21.8%) in dilution 2, 20 samples (23.8%) in dilution 3 and 8 samples (16%) in dilution 4. Penicillium spp. Could be isolated from 36 samples (20%) in dilution 1, 24 samples (18.7%) in dilution 2, 8 samples (9%) in dilution 3 and 0 samples (0%)) in dilution 4. The results of this study indicate the high rate of contamination of broiler feeds to common toxigenic fungi in western parts of Iran.

Keywords


Toxigenic Fungi,Mycotoxins, Broiler Feed, Kermanshah Province.

References