FTIR Spectrum Characteristic of Treated Spent Oil with Fungi

Oil pollution is worldwide major problem in every environment and hence the world is need of a perfect solution for prevention or recovery so, the present investigation was carried out to detect the spent lubricant oil degradation potentional of indigenous fungi in lab level by measuring the growth and analysis the treated sample using FTIR. Soil sample was randomly collected in the different location at railway tracks in Thanjavur junction soil sample are serially and 10^-2 is used for plating technique in PDA medium. After incubation the isolates were obtained and they are Aspergillus terreus, Aspergillus flavus and Aspergillus niger. Oil degrading ability was detected supplying raw spent oil in PDA medium and incoprporated in PD broth at the concentration of (5%.10%, 15% and 20%), detection shows that no fungal cored use raw oil as source of nutrient. In PDA plate's visual detection of zone due to the degradation was noticed. It was maximum by Aspergillus flavus follwed by Aspergillus terreus and Aspergillus niger. In PD broth supplemented with different spent oil concentration (5%,10%,15% and 20%), after 14 days of incubation (30°C), as a visual the biomass of each fungal culture was determined the biomass of different species of fungi in different concentration oil showed growth but with variation the higher mycelial biomass was recorded by Aspergillus flavus. The FT-IR analysis results shows that is major difference in the peak formation between the tested samples which shows that fungal species has utilized or degraded the oil hydrocarbon. Differently as per their metabolize activity.