Determination of Prochlorperazine Maleate and Pyridoxine Hydrochloride in Combined Tablet Dosage form by using First Order Derivative Spectrophotomeric Method

A simple, accurate, economical and reproducible procedure for simultaneous estimation of Prochlorperazine Maleate and Pyridoxine Hydrochloride in their tablet dosage form is described. In this method 0.3M HCl was utilized as solvent. In first order derivative spectroscopic method, two wavelengths 252nm for prochlorperazine maleate (zero cross for pyridoxine hydrochloride) and 228.5nm for pyridoxine hydrochloride (zero cross for prochlorperazine maleate) were selected. Both drugs obey Beer-Lambert's law in concentration range of 1-5 μg/ml (prochlorperazine maleate) and 5-25 μg/ml (pyridoxine hydrochloride) respectively in 0.3M HCl. The suggested method is validated by using ICH validation parameters like accuracy, precision, specificity, ruggdness and linearity. The results of recovery study were found to be within the prescribed limit of 98-102%. During the linearity study it was observed that absorbance values of Prochlorperazine Maleate and Pyridoxine Hydrochloride in marketed formulation were linear in the range of 80% to 120% of test concentration with R² close to 1 for this method of analysis.