Continuous Culture of Acacia mangium Willd

Acacia mangium, is a fast growing leguminous tree. It forms hybrids with A. auriculiformis, by air pollination; thus, micropropagation may provide genetically pure quality planting material. Therefore in vitro cultures were initiated from nodal explants of 1-year-old Mangium plant, on Driver and Kuniyuki medium with 6-benzyl adenine and polyvinyl pyrrolidone. Shoot cultures were further proliferated using on very low level of 6-benzyl adenine. Different adjuvant were tested for shoot multiplication. Shoot cultures were maintained up to 1 ½ years with retaining the shoot proliferation capacity, by normal subculture cycles. These 1 ½ years old shoot cultures were successfully ischolar_mained in vitro on half strength medium in presence of auxin, to regenerate complete plants.