Research Journal of Science and Technology

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Using Jackfruit (Artocarpus heterophyllus) Pulp as Substrate for Cellulase Production by Rhizopus Stolonifer


Affiliations
1 Department of Microbiology, J.J. College of Arts and Science, Pudukkottai.622422, Tamil Nadu, India
2 Department of Botany, Government Arts College, Ariyalur, Tamil Nadu, India
3 PG and Research Department of Microbiology, J.J. College of Arts and Science, Pudukkottai, Tamil Nadu, India
     

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In this present investigation three fungal species namely Rhizopus stolonifer, Penicilium sp Verticillium verticillate were isolated from the ripened Jack fruit (Artocarpus heterophyllus) pulp waste and their cellulase producing ability was screened on Carboxy methyl cellulase agar. Of the three isolates, Rhizopus stolonifer showed maximum cellulase activity. The enzyme production by Rhizopus stolonifer was assayed and the optimum pH, incubation period, temperature, nitrogen source, carbon source were standardized. The fermentation experiments were studied in solid state fermentation (SSF).

Keywords

Jack Fruit Pulp Waste, Solid State Fermentation, Cellulase Enzyme, Rhizopus stolonifer.
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  • Using Jackfruit (Artocarpus heterophyllus) Pulp as Substrate for Cellulase Production by Rhizopus Stolonifer

Abstract Views: 252  |  PDF Views: 1

Authors

T. Kavitha
Department of Microbiology, J.J. College of Arts and Science, Pudukkottai.622422, Tamil Nadu, India
R. Nelson
Department of Botany, Government Arts College, Ariyalur, Tamil Nadu, India
D. Ramesh Babu
PG and Research Department of Microbiology, J.J. College of Arts and Science, Pudukkottai, Tamil Nadu, India

Abstract


In this present investigation three fungal species namely Rhizopus stolonifer, Penicilium sp Verticillium verticillate were isolated from the ripened Jack fruit (Artocarpus heterophyllus) pulp waste and their cellulase producing ability was screened on Carboxy methyl cellulase agar. Of the three isolates, Rhizopus stolonifer showed maximum cellulase activity. The enzyme production by Rhizopus stolonifer was assayed and the optimum pH, incubation period, temperature, nitrogen source, carbon source were standardized. The fermentation experiments were studied in solid state fermentation (SSF).

Keywords


Jack Fruit Pulp Waste, Solid State Fermentation, Cellulase Enzyme, Rhizopus stolonifer.