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Isolation and Biochemical Characterization of Bacteria Present in Roots of Water Hyacinth (Eichhornia crassipes (Mart) Solm.) in Robertson Lake Jabalpur.


Affiliations
1 School of Studies in Life Sciences, Pt. Ravi Shankar Shukla University, Raipur, (C G) 492010, India
2 Department of Biological Sciences, Rani Durgawati University, Jabalpur, India
     

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The present study was conducted to isolate bacteria from ischolar_main of Eichhornia crassipes (water hyacinth). Bacteria were isolated in tryphtone glucose yeast extract agar (TGYEA) medium during different phases of growth of water hyacinth in Robertson lake and on basis of biochemical characteristics probable identification was done by using a PIB program, given by Bryant (1989). A total of 30 bacteria were isolated from ischolar_mains of the growing water hyacinth plants in the Robertson lake Jabalpur. Out of 30 bacteria, 25 were gram - negative rods, comprising of 23 fermentative and 2 non - fermentative, 3 were gram - positive rods, and 2 were gram - positive cocci. Most of the bacteria were able to hydrolyze starch and glucose, and reduce NO3 to NO2, indicating their active role in transformation of C and N. The presence of gelatinase enzyme in most of the bacteria has indicated their role in active degradation of organic N.

Keywords

Water Hyacinth, Bacteria, Aquatic Ecosystem, Robertson Lake, C and N Transformation.
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  • Isolation and Biochemical Characterization of Bacteria Present in Roots of Water Hyacinth (Eichhornia crassipes (Mart) Solm.) in Robertson Lake Jabalpur.

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Authors

Sadhana Jaiswal
School of Studies in Life Sciences, Pt. Ravi Shankar Shukla University, Raipur, (C G) 492010, India
P. K. Singhal
Department of Biological Sciences, Rani Durgawati University, Jabalpur, India

Abstract


The present study was conducted to isolate bacteria from ischolar_main of Eichhornia crassipes (water hyacinth). Bacteria were isolated in tryphtone glucose yeast extract agar (TGYEA) medium during different phases of growth of water hyacinth in Robertson lake and on basis of biochemical characteristics probable identification was done by using a PIB program, given by Bryant (1989). A total of 30 bacteria were isolated from ischolar_mains of the growing water hyacinth plants in the Robertson lake Jabalpur. Out of 30 bacteria, 25 were gram - negative rods, comprising of 23 fermentative and 2 non - fermentative, 3 were gram - positive rods, and 2 were gram - positive cocci. Most of the bacteria were able to hydrolyze starch and glucose, and reduce NO3 to NO2, indicating their active role in transformation of C and N. The presence of gelatinase enzyme in most of the bacteria has indicated their role in active degradation of organic N.

Keywords


Water Hyacinth, Bacteria, Aquatic Ecosystem, Robertson Lake, C and N Transformation.