Optimization of Amylase Production from Bacillus subtilis and Pseudomonas aeruginosa Using Sugarcane Bagasses by Submerged Fermentation

Enzymes are considered as nature’s catalysis most enzymes today (and in future) produced from the microorganisms. In the present study, Bacillus subtilis and Pseudomonas aeruginosa were isolated from the soil samples. The isolated organisms were identified using standard microbiological methods. The inoculums were prepared with waste substrate (sugarcane bagasse). The isolated bacterial strain were inoculated in the medium and incubated at 30°C for 3 days. The high-level amylase production was observed in Bacillus subtilis inoculated medium so this strain used for the optimization studies. The amylase activity was optimized in various physical parameters such as temperature, pH and chemical parameters like nitrogen and carbon sources. The maximum production of amylase was recorded at 35° C and pH 7.0. The amylase production by Bacillus subtilis was recorded maximum in starch-supplemented medium when compared to other carbon sources (maltose and glucose). Among the various nitrogen sources (Ammonium sulphate, ammonium chloride and ammonium nitrate) used, Ammonium sulphate supplemented medium showed the significant results in the production of amylase by Bacillus subtilis.