In Vitro Micropropagation of Colecus arometicus- An Important Medicinal Plant

In vitro micropropagation of Coleus aromaticus using shoot meristems was developed. Coleus arometicus is an important medicinal plant, contains an active ingredient colecus. Multiple shoots were induced in vitro from Apical and Auxiliary meristems. Murashige and Skoog’s medium supplemented with BAP 0.5 to 3.0 mg/l, 2.0 mg/l BAP + 0.5 mg/l Kn, 1.0 mg/l BAP + 1.0 mg/l Kn, 1.0 mg/l BAP + 2.0 mg/l Kn. MS + 2.0 mg/l BAP was found to be most suitable for shoot tip multiplication (75%). For ischolar_maining the shoots were excised and transferred to MS medium combination with ether 10 g/l sucrose or 1 mg/l NAA + 20g sucrose or 200 mg activated charcoal. Rooted plantlets were then transferred to pot covered with polyethylene bags for one week and then grown in the green house. These hardened plants have been successfully established in soil.