Research Journal of Science and Technology

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RP- HPLC Method for Simultaneous Estimation of Ofloxacin and Ornidazole from Bulk and Tablets


Affiliations
1 Dept. of Pharmaceutics, Pravara Rural college of Pharmacy, Pravaranagar, India
2 Sanjivani college of pharmaceutical Education and Research, Kopargaon, India
3 Research Associate Orchid Pharmaceutical Ltd., Chennai, India
     

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A simple, selective, rapid, precise and economical reverse phase highpressure liquid chromatographic method has been developed for the simultaneous estimation of Ofloxacin and Ornidazole from pharmaceutical formulation. The method was carried out on a Kromasil C18 (5μm, 25 cm X 4.6 mm, i.d.) column, with a mobile phase consisting acetonitrile: phosphate buffer (pH 2.4) in the ratio 80: 20% V/V at a flow rate of 1.0 ml/min. Detection was carried out at 294 nm. The retention time of Ofloxacin and Ornidazole were 2.773 and 5.448 min respectively. The developed method was validated in terms of accuracy, precision, linearity, Limit of detection, Limit of quantitation. The proposed method can be used for estimation of these drugs in combined dosage form for routine analysis.

Keywords

Ofloxacin, Ornidazole, RP-HPLC.
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  • RP- HPLC Method for Simultaneous Estimation of Ofloxacin and Ornidazole from Bulk and Tablets

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Authors

R. S. Jadhav
Dept. of Pharmaceutics, Pravara Rural college of Pharmacy, Pravaranagar, India
P. N. Kendre
Sanjivani college of pharmaceutical Education and Research, Kopargaon, India
M. H. Kolhe
Dept. of Pharmaceutics, Pravara Rural college of Pharmacy, Pravaranagar, India
S. N. Lateef
Sanjivani college of pharmaceutical Education and Research, Kopargaon, India
S. M. Shelke
Research Associate Orchid Pharmaceutical Ltd., Chennai, India
R. K. Godge
Dept. of Pharmaceutics, Pravara Rural college of Pharmacy, Pravaranagar, India

Abstract


A simple, selective, rapid, precise and economical reverse phase highpressure liquid chromatographic method has been developed for the simultaneous estimation of Ofloxacin and Ornidazole from pharmaceutical formulation. The method was carried out on a Kromasil C18 (5μm, 25 cm X 4.6 mm, i.d.) column, with a mobile phase consisting acetonitrile: phosphate buffer (pH 2.4) in the ratio 80: 20% V/V at a flow rate of 1.0 ml/min. Detection was carried out at 294 nm. The retention time of Ofloxacin and Ornidazole were 2.773 and 5.448 min respectively. The developed method was validated in terms of accuracy, precision, linearity, Limit of detection, Limit of quantitation. The proposed method can be used for estimation of these drugs in combined dosage form for routine analysis.

Keywords


Ofloxacin, Ornidazole, RP-HPLC.