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Actinomycetes Synthesized Nanoparticles and their Antibacterial Activity


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1 Medical Laboratory Science, Dilla University and Referral Hospital, Ethiopia
     

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One Gram negative bacterium (Bacillus subtilis) and two Gram positive bacteria (Pseudomonas aeruginosa and Escherichia coli) were tested against silver nanoparticles synthesized (Nps) by soil isolated actinomycetes for its antibacterial activities. The zone of inhibition of silver nanoparticles was measured. The results indicated that the silver nanoparticles synthesized from Actinomycetes showed effective antibacterial activity against all the test organisms. Among the three test organisms tested P. aeruginosa showed to be more susceptible (16 mm) against the Nps synthesized with a quantity of 40 μl followed by B.subtilis (11 mm) and E.coli (11 mm) with the same concentration of Nps. Previously the synthesized Nps were confirmed for their existence with the help of UV-Vis, SEM and FTIR. The actinomycetes synthesized Nps were found to be effective enough against pathogenic organisms and can be synthesized in a large scale which needed further research and analysis.

Keywords

Actinomycetes, Silver Nitrate, Nanoparticles, Antibacterial.
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  • Gaikwad Sagar and Bhosale Ashok, (2012). Green Synthesis of Silver Nanoparticles Using Aspergillus niger and Its Efficacy Against Human Pathogens. Padmashri Vikhe Patil College. European J. of Experimental biology, 2 (5):1654-1658.

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  • Actinomycetes Synthesized Nanoparticles and their Antibacterial Activity

Abstract Views: 306  |  PDF Views: 8

Authors

P. Danial Thomas
Medical Laboratory Science, Dilla University and Referral Hospital, Ethiopia

Abstract


One Gram negative bacterium (Bacillus subtilis) and two Gram positive bacteria (Pseudomonas aeruginosa and Escherichia coli) were tested against silver nanoparticles synthesized (Nps) by soil isolated actinomycetes for its antibacterial activities. The zone of inhibition of silver nanoparticles was measured. The results indicated that the silver nanoparticles synthesized from Actinomycetes showed effective antibacterial activity against all the test organisms. Among the three test organisms tested P. aeruginosa showed to be more susceptible (16 mm) against the Nps synthesized with a quantity of 40 μl followed by B.subtilis (11 mm) and E.coli (11 mm) with the same concentration of Nps. Previously the synthesized Nps were confirmed for their existence with the help of UV-Vis, SEM and FTIR. The actinomycetes synthesized Nps were found to be effective enough against pathogenic organisms and can be synthesized in a large scale which needed further research and analysis.

Keywords


Actinomycetes, Silver Nitrate, Nanoparticles, Antibacterial.

References