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Cytogenetic Study of Non-Permitted Food Dye-Lead Chromate in Swiss Albino Mice


Affiliations
1 Department of Zoology, University of Rajasthan, Jaipur, India
2 S.S. Jain Subodh P.G. College, Jaipur, India
     

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The current wave of appalling shortage and scarcity of food and agricultural produce throughout the world has resulted in the spiraling prices of eatables. Implicit in the situation is another serious problem of adulteration of food, which has recently made its dreadful appearance on a scale rarely witnessed in the past. Among various contaminants of food, the use of food colours, in particular, have attained alarming dimensions in the recent years. It is a matter of serious concerns that in spite of regulatory surveillance the use of non-permitted colours in some loose/non-branded products in both rural and urban markets is continuing. Majority of non-permitted food dyes such as Metanil yellow, Orange II, Rhodamine B, Malachite green, Auramine, Amaranth and Sudan dyes are known to cause varied toxic manifestations in the experimental animals. The present study was aimed to evaluate the cytogenetic effects of a non-permitted food dye lead chromate on swiss albino mice. The results clearly indicate the chromotoxic effects of lead chromate, both for short term and long-term experiments.


Keywords

Non-Permitted, Lead Chromate, Albino Mice, Amaranth.
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  • Alves D. R., Azevedo L., Ribeiro L. R. and Salvadori D. M.: Study on the mutagenicity and antimutagenicity of a natural food colour (Annatto) in mouse bone marrow cells. Food Chem. Toxicol.41: pp 189-192. 2003.

  • Amerine M. A., Pangborn R. M. and Roessler E. B.: Principles of sensory evaluation of food. Academic Press, New York. 1965: pp 53-66.

  • Ashfaq N. and Masud T: Surveillance on artificial colours in different ready to eat foods. Pak. J. Nutritn.1: pp 223-225 2002

  • Babu S. and Shenoliker I. S.: Health and nutritional implications of food colours. Ind. J. Med. Res.102: pp 245-249 1995.

  • Bhat R. V. and Mathur P.: Changing scenario of food colours in India. Curr. Sci.74: pp 198-202. 1998.

  • Chauhan L. K. S. and Sundararaman V.: Effects of substituted ureas on plant cells. I. Cytological effects of Isopruturon on the ischolar_main meristem cells of Allium cepa. Cytologia.55: pp 91-98, 1990

  • Dhatchayani M., Jayaraman K. and Raghwan L.: Genotoxic study on the effects of Indigo carmine (a food colour) in a mammalian system. Ind. J. Toxicol.9: pp 101-106. 2002

  • Dixit S., Pandey R. C., Das M. and Khanna S. K.: Food quality surveillance on colours in eatables sold in rural markets of Uttar Pradesh. J. Food Sci. Technol.32: pp 373-376, 1995

  • Downham A. and Collins P.: Colouring our foods in the last and next millennium. Int. J. Food Sci. Technol.35: pp 5-22, 2000

  • Epel D. (1963) Giri A. K., Banerjee T. S., Talukdar G. and Sharma A.: Effects of dyes (Indigo carmine, Metanil yellow and Fast green FCF) and Nitrite in vivo on bone marrow chromosomes of mice. Cancer Lett.30: pp 315-320, 1986 a

  • Giri A. K., Talukdar G. and Sharma A : Sister chromatid exchange induced by Metanil yellow and Nitrite singly and in combination in vivo on mice. Cancer Lett. 31: pp299-303, 1986 b

  • Giri A. K., Mukherjee A., Talukdar G. and Sharma A. In vivo cytogenetic studies on mice exposed to Orange G, a food colourant. Toxicol.Lett.,44: pp 253-261, 1988

  • Giri A. K.: Food dyes of India: Mutagenic and clastogenic potential - A Review. Proc. Ind. Natl. Sci. Acad. 57: pp 183-198, 1991

  • Grant W. F: Chromosome aberration in plant as monitoring system. Environ. Hlth. Perspect.27: pp 37-43, 1978

  • Gomurgen A. N.: Cytological effect of the Potassium metabisulphite and Potassium nitrate food preservative on ischolar_main tips of Allium cepa L.Cytologia.70: pp 119-128, 2005

  • Hasegawa M. M., Nishi Y., Ohkawa Y. andInui N.: Effects of Sorbic acid and its salts on chromosome aberrations, sister chromatid exchanges and gene mutations in cultured Chinese hamster cells. Food ChemToxicol.22: pp 501-507, 1984

  • Holmes A. L., Wise S. S., Sandwick S. J., Lingle W. L., Negron V. C., Thompson W. D. and Wise J. P. Sr : Chronic exposure to Lead chromate causes centrosome abnormalities and aneuploidy in human lung cells. Cancer Res. 66: pp 4041-4048, 2006

  • Ipsen J. and Feigl P. : Bancroft’s introduction to biostatistics.2nd ed. Harper and Row Publishers Inc., New York. 1970, pp 1-223.

  • Jain A. K. and Andsorbhoy R. K.: Cytogenetical studies on the effects of some Chlorinated pesticides III. Cytologia.53: pp 427-436, 1988

  • Khanna S. K., Singh G. B. and Singh S. B.: Non-permitted colours in food and their toxicity. J. Food Sci. Technol.10: pp 33-36, 1973

  • Khanna S. K., Singh G. B. and Singh S. B.: Adulterants in our daily eatables and their toxicity. Ind. J. Pub. Hlth. 19: pp 59, 1975

  • Khanna S. K., Srivastava L. P. and Singh G. B.: Toxicity studies on Metanil yellow in rats. Environ. Res.15: pp 227-231, 1978

  • Khanna S. K., Singh G. B. and Krishnamurthi C. R.: Toxicity profile of some commonly encountered food colours. J. Food Sci. Technol.10: pp 95-103, 1980

  • Khanna S. K., Singh G. B. and Dixit A. K.: Use of synthetic dyes in eatables of rural areas. J. Food Sci. Technol. 22: pp 269-272, 1985

  • Khanna S. K., Srivastava L. P. and Singh G. B.: Comparative usage pattern of synthetic dyes in Yellow orange coloured eatables among urban and rural markets. Indian Food Paker.40: pp 33-37, 1986

  • Khanna S. K. and Das M.: Toxicity, carcinogenicity and clinicoepidemiologic studies on dyes and dye intermediates. J. Sci. Industr. Res.50: pp 965-974, 1991

  • Kihlman B. A. and Natarajan A. T.: Potentiation of chromosomal alterations by inhibitors of DNA repair. In: DNA repair and its inhibition (Collins A., Dowens C. S. and Johson R. T. ed.). IRL, Oxford. pp 319-339, 1984

  • Mathur P.: Risk analysis of food adulterants. Ph.D. thesis submitted to University of Delhi, New Delhi. 2000

  • Njagi G. D. and Gopalan H. N.: Cytogenetic effects of the food preservative Sodium benzoate and Sodium sulphite on Viciafaba ischolar_main meristem. Mutat. Res. 102: pp 213-219, 1982

  • Noviello E., Alvigi M., Cimoli G., Rovini E., Mazzoni A., Porodi S., De Sessa F. and Russo P.: Sister chromatid exchange, chromosome aberrations and cytotoxicity produced by topoisomerase II targeted drugs in sensitive (A2780) and resistant (A2780-DX3) human ovarian cancer cells: correlations with the formation of DNA double-strand breaks. Mutat. Res.311: pp 21-29, 1994

  • Ollikainen T. H.: Pleasantness of colourless and decoloured soft drinks and consumers attitudes to artificial food colours. Appetite.3: pp 369-373, 1982

  • PFAA: Prevention of Food Adulteration Act, Rule.16th ed. Indian Eastern Book Company. 1954

  • PFA: Prevention of Food Adulteration Act of India, 1954 and Rules. Lucknow, India: Indian Eastern Book Company. 2003

  • Prasad O. and Rastogi P. B.: Orange II induced cytogenetical changes in albino mice. Cell. Mol. Life Sci.38: pp 1240-1242, 1982

  • Rastogi P. B., Thilly W. G. and Shirname-More L: Long-term low-dose mutation studies in human cells: Metanil yellow and Orange II. Mutat. Res. 249: pp 265-273, 1991

  • Recunzogullari E., Kayraldiz A., Ila H. B., Cakmak T. and Topaktas M: The cytogenetic effects of Sodium metabisulphite, a food preservative in ischolar_main tip cells of Allium cepa. Turk. J. Biol. 25: pp 361-370, 2001

  • Recunzogullari E., Tuytu B. A., Topaktas M., Basrilla H., Kayraediz A., Arsean M. and Diler S. B.: Genotoxicity of Aspartame. Drug Chem. Toxicol.27: pp 257-268. (2004)

  • Roy A. K. and Chakraborti J.: Added colours in foodstuffs from Calcutta markets. Sci. Culture.57: pp 40-41, 1991

  • Singh R. L., Khanna S. K. and Singh G. B.: Acute and short term toxicity studies in Orange II. Vet. Hum. Toxicol.29: pp 300-304, 1987

  • Singh R. L., Khanna S. K. and Singh G. B.: Acute and short term toxicity of a popular blend of Metanil yellow and Orange II in albino rats. Ind. J. Exp. Biol.26: pp 105-111, 1988.

  • Srivastava S. J., Sinha R. and Roy D.: Toxicological effects of Malachite green: A review. Aquat.Toxicol.66: pp 319-329,2004

  • Turkoglu S.: Genotoxicity of five food preservatives tested on ischolar_main tips of Allium cepa L. Mut. Res.626: pp 4-14,2007

  • Vaidya V. G. and Godbole N. N.: Effect of Metanil yellow on human leucocyte chromosome in vitro. Ind. J. Exp. Biol.16: pp 820-821, 1978

  • Watanbe M., Takayama Y., Koike M. and Yamamoto M.: In vivo clastogenecity of Lead chromate in mice. Tohoku J. Exp. Med. 146: pp 373-374, 1985

  • Wess J. A. and Archer D. C.: Disparate in vivo and in vitro immunomodulatory activities of Rhodamine B. Food Chem. Toxicol. 20: pp 9-14, 1982


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  • Cytogenetic Study of Non-Permitted Food Dye-Lead Chromate in Swiss Albino Mice

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Authors

Geetanjali Chakravarty
Department of Zoology, University of Rajasthan, Jaipur, India
Shipra Sharma
S.S. Jain Subodh P.G. College, Jaipur, India
Preeti Srivastava
S.S. Jain Subodh P.G. College, Jaipur, India
Yashoda Saini
S.S. Jain Subodh P.G. College, Jaipur, India

Abstract


The current wave of appalling shortage and scarcity of food and agricultural produce throughout the world has resulted in the spiraling prices of eatables. Implicit in the situation is another serious problem of adulteration of food, which has recently made its dreadful appearance on a scale rarely witnessed in the past. Among various contaminants of food, the use of food colours, in particular, have attained alarming dimensions in the recent years. It is a matter of serious concerns that in spite of regulatory surveillance the use of non-permitted colours in some loose/non-branded products in both rural and urban markets is continuing. Majority of non-permitted food dyes such as Metanil yellow, Orange II, Rhodamine B, Malachite green, Auramine, Amaranth and Sudan dyes are known to cause varied toxic manifestations in the experimental animals. The present study was aimed to evaluate the cytogenetic effects of a non-permitted food dye lead chromate on swiss albino mice. The results clearly indicate the chromotoxic effects of lead chromate, both for short term and long-term experiments.


Keywords


Non-Permitted, Lead Chromate, Albino Mice, Amaranth.

References