Development and Evaluation of Cosmeceutical Nanolipogel

Ageing is one of the major problems and the telltale sign of ageing is increased wrinkling of the face along with loss of moisture from the skin, making skin dry and scaly. Exposure of the skin to UV light also causes degenerative effects; this can be minimized by using antioxidants like vitamins A, C or E. Thus the problem of wrinkle and dry skin can be solved using antiwrinkle agent along with vitamins. However, barrier properties of the skin limit their use. Therefore in the present study, BoNT-L-Peptide (INCI name- palmitoyl hexapeptide, 50 ppm solution) and vitamin E acetate was encapsulated into liposome for improving its topical delivery. Liposomes were prepared ethanol injection method and optimized by factorial design approach. Liposomes were characterized for pH, vesicle size, zeta potential, drug content and drug release. Further gels were prepared by using stable liposomal dispersion using carbopol (carbomer) U21, this gel was called as lipogel and were characterized for pH, spreadability, viscosity, drug content and drug release. Stability of liposomal dispersion and lipogel was studied at 25°C/60% RH, 30°C/65% RH&40°C/75% RH as per ICH guidelines. Stable and homogeneous liposomal dispersion and lipogel were developed. The drug content was found in the range of 97 % - 101 %, with the particle size distribution between 159.6( ±0.22) nm to 239.5 (±0.33) nm. Controlled drug release was found to be upto 24 hours at pH 6.8.