South Indian Journal of Biological Sciences

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TALENs, a Revolutionary Innovation Tool in Germline Genome-Editing


Affiliations
1 Aquaculture Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore, 632 014 Tamil Nadu, India
2 Laboratory of Infectious Disease, College of Veterinary Medicine, Chungnam National University, Daejeon, 305‐764, Korea, Republic of
3 Department of Genetics, King Faisal Specialist Hospital and Research Center (KFSH&RC), MBC‐03, PO BOX 3354, Riyadh, 11211, Saudi Arabia
 

Transcription activator‐like effector nuclease (TALENs) is an emerging programmable nuclease, enable precise genome editing by introducing DNA double‐strand breaks (DSBs) at specific genomic loci. DSBs subsequently recruit the endogenous non‐homologous end‐joining (NHEJ) or homology‐directed repair (HDR) units to the DSB site to mediate genome editing. Mutagenesis induced by the TALENs have shown higher efficacy, as much as 76%, and have been demonstrated to induce effective and heritable mutations in various organisms and cells, thus proving furthermore, to be highly scalable and reliable and is an applicable method to single cells and multicellular organisms. In this mini‐review, we summarized recent findings and applications of TALEN mediated germ line knock‐down in cells and vertebrate animal models.

Keywords

TALENs, DBS, Mutagenesis, HDR and NHEJ.
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  • TALENs, a Revolutionary Innovation Tool in Germline Genome-Editing

Abstract Views: 181  |  PDF Views: 6

Authors

Maivannan Thiruvarangan
Aquaculture Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore, 632 014 Tamil Nadu, India
Maheswaran Easwaran
Laboratory of Infectious Disease, College of Veterinary Medicine, Chungnam National University, Daejeon, 305‐764, Korea, Republic of
Raja Sudhakaran
Aquaculture Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore, 632 014 Tamil Nadu, India
Sateesh Maddirevula
Department of Genetics, King Faisal Specialist Hospital and Research Center (KFSH&RC), MBC‐03, PO BOX 3354, Riyadh, 11211, Saudi Arabia

Abstract


Transcription activator‐like effector nuclease (TALENs) is an emerging programmable nuclease, enable precise genome editing by introducing DNA double‐strand breaks (DSBs) at specific genomic loci. DSBs subsequently recruit the endogenous non‐homologous end‐joining (NHEJ) or homology‐directed repair (HDR) units to the DSB site to mediate genome editing. Mutagenesis induced by the TALENs have shown higher efficacy, as much as 76%, and have been demonstrated to induce effective and heritable mutations in various organisms and cells, thus proving furthermore, to be highly scalable and reliable and is an applicable method to single cells and multicellular organisms. In this mini‐review, we summarized recent findings and applications of TALEN mediated germ line knock‐down in cells and vertebrate animal models.

Keywords


TALENs, DBS, Mutagenesis, HDR and NHEJ.

References