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In Vitro Anti Oxidant Activity of Chromatographically Separated Fractions from the Leaves of Aastilbe rivularis Buch. - Ham. Ex D. Don


Affiliations
1 Department of Medical Biotechnology, Sikkim Manipal University, Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, India
2 Department of Biochemistry, Sikkim Manipal University, Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, India
 

In vitro antioxidant activity of chromatographically separated fractions from powdered leaves of Aastilbe rivularis buch. - Ham. Ex D. Don was studied. Antioxidant activity was measured by superoxide anion generation with help of xanthine-xanthine oxidase assay and with linoleic acid peroxidation assay as well as DPPH photometric assay. Total phenol, flavonoids, ascorbic acid and carotenoid contents in the separated fractions were also estimated. Out of five fractions separated, the third fraction had maximuum antioxidant activity which was comparable to that of known synthetic antioxidant. Antioxidant activity was related with high content of total phenol, flavonoid, ascorbic acid and carotenoids in the fraction.

Keywords

Astilbe rivularis, Buch. - Ham. Ex D. Don, Chromatographic Separation, Anti Oxidant Activity, Total Phenol, Flavonoid, Ascorbic Acid.
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  • In Vitro Anti Oxidant Activity of Chromatographically Separated Fractions from the Leaves of Aastilbe rivularis Buch. - Ham. Ex D. Don

Abstract Views: 199  |  PDF Views: 158

Authors

Prasanta Kumar Mitra
Department of Medical Biotechnology, Sikkim Manipal University, Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, India
Tanaya Ghosh
Department of Medical Biotechnology, Sikkim Manipal University, Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, India
Prasenjit Mitra
Department of Biochemistry, Sikkim Manipal University, Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, India

Abstract


In vitro antioxidant activity of chromatographically separated fractions from powdered leaves of Aastilbe rivularis buch. - Ham. Ex D. Don was studied. Antioxidant activity was measured by superoxide anion generation with help of xanthine-xanthine oxidase assay and with linoleic acid peroxidation assay as well as DPPH photometric assay. Total phenol, flavonoids, ascorbic acid and carotenoid contents in the separated fractions were also estimated. Out of five fractions separated, the third fraction had maximuum antioxidant activity which was comparable to that of known synthetic antioxidant. Antioxidant activity was related with high content of total phenol, flavonoid, ascorbic acid and carotenoids in the fraction.

Keywords


Astilbe rivularis, Buch. - Ham. Ex D. Don, Chromatographic Separation, Anti Oxidant Activity, Total Phenol, Flavonoid, Ascorbic Acid.