In vitro antioxidant activity of chromatographically separated fractions from powdered leaves of Aastilbe rivularis buch. - Ham. Ex D. Don was studied. Antioxidant activity was measured by superoxide anion generation with help of xanthine-xanthine oxidase assay and with linoleic acid peroxidation assay as well as DPPH photometric assay. Total phenol, flavonoids, ascorbic acid and carotenoid contents in the separated fractions were also estimated. Out of five fractions separated, the third fraction had maximuum antioxidant activity which was comparable to that of known synthetic antioxidant. Antioxidant activity was related with high content of total phenol, flavonoid, ascorbic acid and carotenoids in the fraction.
Keywords
Astilbe rivularis, Buch. - Ham. Ex D. Don, Chromatographic Separation, Anti Oxidant Activity, Total Phenol, Flavonoid, Ascorbic Acid.
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