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New Method for Spectrophotometric Determination of Lidocaine


Affiliations
1 Department of Chemistry, Techreen University, Lattakia, Syrian Arab Republic
 

This work include prepration the organic reagent 3-hydroxy-4-[(4-hydroxyphenyl)azo]-1- naphthalenesulfonic acid by coupling diazonium of 1-amino-2-naphthol-4-sulfonic acid with phenol. The reagent react with Lidocaine in acidic medium( PH = 4) to produce red colored complex with mole ratio 1:1 (L:M) and maximum absorbance at 510 nm . this method show obey to beers in the range of 1.44 - 69.31 ppm, with molar absorptivity of 4.1633×103 L mole-1 cm-1 Sandell's sensitivity 0.0693 μg·cm-2 The effects of variables such as pH, time, concentration of color producing reagent, and stability of color were investigated to optimize the procedure. The results are validated statistically. The proposed method was applied to commercially available tablets, and the results were Pharmaceutical formulations.

Keywords

Lidocaine, Determination, Spectrophotometry.
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  • New Method for Spectrophotometric Determination of Lidocaine

Abstract Views: 453  |  PDF Views: 183

Authors

Malek M. S. Okdeh
Department of Chemistry, Techreen University, Lattakia, Syrian Arab Republic
Chahid Moustpha
Department of Chemistry, Techreen University, Lattakia, Syrian Arab Republic
Mohammad Shraitah
Department of Chemistry, Techreen University, Lattakia, Syrian Arab Republic

Abstract


This work include prepration the organic reagent 3-hydroxy-4-[(4-hydroxyphenyl)azo]-1- naphthalenesulfonic acid by coupling diazonium of 1-amino-2-naphthol-4-sulfonic acid with phenol. The reagent react with Lidocaine in acidic medium( PH = 4) to produce red colored complex with mole ratio 1:1 (L:M) and maximum absorbance at 510 nm . this method show obey to beers in the range of 1.44 - 69.31 ppm, with molar absorptivity of 4.1633×103 L mole-1 cm-1 Sandell's sensitivity 0.0693 μg·cm-2 The effects of variables such as pH, time, concentration of color producing reagent, and stability of color were investigated to optimize the procedure. The results are validated statistically. The proposed method was applied to commercially available tablets, and the results were Pharmaceutical formulations.

Keywords


Lidocaine, Determination, Spectrophotometry.

References