The Design of Scaffolds for Use in Tissue Engineering

Problems: Tissue engineering (TE) is one of the modern strategies to provide a functional biological tissue equivalent to restore or improve the function of human tissues that lost by disease, traumatic events, or congenital abnormalities. Some essential factors in TE are tissue type and appropriate scaffold. suitable influence on cell viability and proliferation is One of the most important characteristics of a appropriate scaffold for tissue engineering. The purpose of this research was to comparative evaluation of adipose derived stem cells (ADSCs) proliferation rate and their viability on the five different scaffolds. Experimental approach: six different scaffolds were prepared including Alginate, Poly Lactic Glycolic Acid (PLGA), Fibrin glue (FG), Inactive Platelet-Rich Plasma (IPRP), Active Platelet-Rich Plasma (APRP) and Hydroxyapatite/β-tricalcium phosphate (HA/β-TCP). Human adipose tissue was applied as mesenchymal stem cells source. These cells were seeded in the targeted scaffolds. After 48 hours, the proliferation and viability of mesenchymal stem cells were assessed by MTT assay. The obtained results were analyzed statistically by SPSS software (p<0.05). Findings: Significant differences between cell proliferation and viability of different scaffolds have been shown. Alginate and active PRP were shown to be the most and least suitable scaffolds in terms of enhancing cell proliferation and maintaining cell viability respectively (p60;0.05). Conclusion: Our study indicated that active PRP could be the best scaffold for support of Adipose-derived stem cells proliferation in comparison with others.