Journal of Pure and Applied Ultrasonics

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Synergistic Effect of Ultrasound Assisted Extraction and Two-phase Systems on the Recovery of Penicillin G from Water


Affiliations
1 Université Blida 1, Faculté de Technologie, Laboratoire de Génie Chimique (LGC), B.P. 270, Route de Soumaa, 09000 Blida, Algérie., Algeria
2 Institut UTINAM UMR 6213 CNRS Université Bourgogne Franche-Comté, 30 avenue de l'Observatoire, 25009 Besançon cedex France., France
 

Penicillin G (Peni G) is a molecule of high interest for pharmaceutical use, but a purification process is required. In this work, phase diagrams of aqueous two-phase systems (ATPS) containing Polyethylene glycol (PEG-6000) - Sodium citrate - water as well as the partitioning behaviour of penicillin G in these systems are presented. The effect of different parameters on extraction conditions such as salt (type and concentration), polyethylene glycol molecular weight and concentration, temperature, pH of buffer solution, volume ratio and the effect of ultrasound were evaluated and optimized. Aqueous two-phase systems of PEG-6000 37.5% (w/w)/ citrate buffer solution 28.5% (w/w) with a ratio of 2/1, pH 6.16, and at room temperature (22°C), assisted by ultrasound (20 kHz value of frequency) is shown to be an effective way of separating and concentrating penicillin G from an aqueous phase and also gives a reduced consumption of time and organic solvents. In those conditions, separation efficiency is upper than 99% compared with liquid/liquid extraction 85%.

Keywords

Partitioning, Phase-diagrams, Polyethylene Glycol, Separation, Sodium Citrate
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  • Synergistic Effect of Ultrasound Assisted Extraction and Two-phase Systems on the Recovery of Penicillin G from Water

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Authors

Aicha Ladaidi
Université Blida 1, Faculté de Technologie, Laboratoire de Génie Chimique (LGC), B.P. 270, Route de Soumaa, 09000 Blida, Algérie., Algeria
Hocine Boutoumi
Université Blida 1, Faculté de Technologie, Laboratoire de Génie Chimique (LGC), B.P. 270, Route de Soumaa, 09000 Blida, Algérie., Algeria
Hussein Khalaf
Université Blida 1, Faculté de Technologie, Laboratoire de Génie Chimique (LGC), B.P. 270, Route de Soumaa, 09000 Blida, Algérie., Algeria
Loic Hallez
Institut UTINAM UMR 6213 CNRS Université Bourgogne Franche-Comté, 30 avenue de l'Observatoire, 25009 Besançon cedex France., France
Jean-Yves Hihn
Institut UTINAM UMR 6213 CNRS Université Bourgogne Franche-Comté, 30 avenue de l'Observatoire, 25009 Besançon cedex France., France
Marielle Remery
Institut UTINAM UMR 6213 CNRS Université Bourgogne Franche-Comté, 30 avenue de l'Observatoire, 25009 Besançon cedex France., France

Abstract


Penicillin G (Peni G) is a molecule of high interest for pharmaceutical use, but a purification process is required. In this work, phase diagrams of aqueous two-phase systems (ATPS) containing Polyethylene glycol (PEG-6000) - Sodium citrate - water as well as the partitioning behaviour of penicillin G in these systems are presented. The effect of different parameters on extraction conditions such as salt (type and concentration), polyethylene glycol molecular weight and concentration, temperature, pH of buffer solution, volume ratio and the effect of ultrasound were evaluated and optimized. Aqueous two-phase systems of PEG-6000 37.5% (w/w)/ citrate buffer solution 28.5% (w/w) with a ratio of 2/1, pH 6.16, and at room temperature (22°C), assisted by ultrasound (20 kHz value of frequency) is shown to be an effective way of separating and concentrating penicillin G from an aqueous phase and also gives a reduced consumption of time and organic solvents. In those conditions, separation efficiency is upper than 99% compared with liquid/liquid extraction 85%.

Keywords


Partitioning, Phase-diagrams, Polyethylene Glycol, Separation, Sodium Citrate

References