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Polymerase Chain Reaction Detection of Genes Responsible for Multiple Antibiotic Resistance Staphylococcus aureus Isolated from Food of Animal Origin in Egypt


Affiliations
1 Department of Bacteriology, Mycology and Immunology, Beni-Suef University, Beni-Suef, Egypt
2 Department of Microbiology and Immunology, National Research Center, Cairo, Egypt
 

Aim: The aim of our study was polymerase chain reaction (PCR) detection of the genes responsible for the multiple antibiotic resistance S. aureus isolated from food of animal origin in Egypt.
Materials and Methods: A total of 125 samples were randomly collected from milk, meat, and their products from Giza and Beni-Suef Governorates markets. The S. aureus isolates were subjected to antimicrobial sensitivity tests using four antibacterial disks (Oxoid), and then the polymerase chain reaction (PCR) was performed for detection of antibiotic resistance genes.
Results: Out of 125 samples, 19 S. aureus isolates were detected. All detected isolates were multiple drug resistance (MDR). The penicillin-, erythromycin-, kanamycin-, and tetracycline-resistant isolates were examined by PCR for resistance genes blaZ, (msrA, ermB, and ermC), aac(6’)aph (2’’), and tetK. The isolates harbored these resistance genes with percentage of 100% (100%, 0%, and 100%), 62.5%, and 100%, respectively.
Conclusion: Contaminated foods of animal origin may represent a source of MDR S. aureus that can be a major threat to public health.

Keywords

Food of Animal Origin, Multiple Antibiotic Resistance, Polymerase Chain Reaction, Resistance Genes, Staphylococcus aureus.
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  • Polymerase Chain Reaction Detection of Genes Responsible for Multiple Antibiotic Resistance Staphylococcus aureus Isolated from Food of Animal Origin in Egypt

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Authors

Fawzy R. El Seedy
Department of Bacteriology, Mycology and Immunology, Beni-Suef University, Beni-Suef, Egypt
A. A. Samy
Department of Microbiology and Immunology, National Research Center, Cairo, Egypt
Hala S. H. Salam
Department of Bacteriology, Mycology and Immunology, Beni-Suef University, Beni-Suef, Egypt
Eman A. Khairy
Department of Microbiology and Immunology, National Research Center, Cairo, Egypt
Aya A. Koraney
Department of Microbiology and Immunology, National Research Center, Cairo, Egypt

Abstract


Aim: The aim of our study was polymerase chain reaction (PCR) detection of the genes responsible for the multiple antibiotic resistance S. aureus isolated from food of animal origin in Egypt.
Materials and Methods: A total of 125 samples were randomly collected from milk, meat, and their products from Giza and Beni-Suef Governorates markets. The S. aureus isolates were subjected to antimicrobial sensitivity tests using four antibacterial disks (Oxoid), and then the polymerase chain reaction (PCR) was performed for detection of antibiotic resistance genes.
Results: Out of 125 samples, 19 S. aureus isolates were detected. All detected isolates were multiple drug resistance (MDR). The penicillin-, erythromycin-, kanamycin-, and tetracycline-resistant isolates were examined by PCR for resistance genes blaZ, (msrA, ermB, and ermC), aac(6’)aph (2’’), and tetK. The isolates harbored these resistance genes with percentage of 100% (100%, 0%, and 100%), 62.5%, and 100%, respectively.
Conclusion: Contaminated foods of animal origin may represent a source of MDR S. aureus that can be a major threat to public health.

Keywords


Food of Animal Origin, Multiple Antibiotic Resistance, Polymerase Chain Reaction, Resistance Genes, Staphylococcus aureus.