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Virulence Gene Profiles of Arcobacter Species Isolated From Animals, Foods of Animal Origin, and Humans in Andhra Pradesh, India


Affiliations
1 Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
2 Department of Veterinary Microbiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
3 Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, India
 

Aim: This study aimed to detect putative virulence genes in Arcobacter species of animal and human origin.
Materials and Methods: A total of 41 Arcobacter isolates (16 Arcobacter butzleri, 13 Arcobacter cryaerophilus, and 12 Arcobacter skirrowii) isolated from diverse sources such as fecal swabs of livestock (21), raw foods of animal origin (13), and human stool samples (7) were subjected to a set of six uniplex polymerase chain reaction assays targeting Arcobacter putative virulence genes (ciaB, pldA, tlyA, mviN, cadF, and cj1349).
Results: All the six virulence genes were detected among all the 16 A. butzleri isolates. Among the 13 A. cryaerophilus isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 61.5, 84.6, 76.9, 76.9, 61.5, and 61.5% of isolates, respectively. Among the 12 A. skirrowii isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 50.0, 91.6, 83.3, 66.6, 50, and 50% of isolates, respectively.
Conclusion: Putative virulence genes were detected in majority of the Arcobacter isolates examined. The results signify the potential of Arcobacter species as an emerging foodborne pathogen.

Keywords

Arcobacter, Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii, Polymerase Chain Reaction, Virulence Genes.
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  • Virulence Gene Profiles of Arcobacter Species Isolated From Animals, Foods of Animal Origin, and Humans in Andhra Pradesh, India

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Authors

M. Soma Sekhar
Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
S. R. Tumati
Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
B. K. Chinnam
Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
V. S. Kothapalli
Department of Veterinary Microbiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
N. Mohammad Sharif
Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, India

Abstract


Aim: This study aimed to detect putative virulence genes in Arcobacter species of animal and human origin.
Materials and Methods: A total of 41 Arcobacter isolates (16 Arcobacter butzleri, 13 Arcobacter cryaerophilus, and 12 Arcobacter skirrowii) isolated from diverse sources such as fecal swabs of livestock (21), raw foods of animal origin (13), and human stool samples (7) were subjected to a set of six uniplex polymerase chain reaction assays targeting Arcobacter putative virulence genes (ciaB, pldA, tlyA, mviN, cadF, and cj1349).
Results: All the six virulence genes were detected among all the 16 A. butzleri isolates. Among the 13 A. cryaerophilus isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 61.5, 84.6, 76.9, 76.9, 61.5, and 61.5% of isolates, respectively. Among the 12 A. skirrowii isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 50.0, 91.6, 83.3, 66.6, 50, and 50% of isolates, respectively.
Conclusion: Putative virulence genes were detected in majority of the Arcobacter isolates examined. The results signify the potential of Arcobacter species as an emerging foodborne pathogen.

Keywords


Arcobacter, Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii, Polymerase Chain Reaction, Virulence Genes.