Indian Journal of Bioinformatics and Biotechnology

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Diversity of 2, 4 Dichlorobiphenyl Degrading Consortium of Pseudomonas Isolates GSa and GSb for Degradation of Poly Chlorinated Biphenyl Congeners


Affiliations
1 Department of Microbiology, Davangere University, Shivagangothri, Davangere-577002, India
 

Objective: Polychlorinated biphenyls (PCB) are persistent organic pollutants that are widely distributed in the environment. PCBs are aromatic compounds have more than 210 congeners, nonvolatile, chemically inert and do not undergo oxidation, reduction or addition reactions, elimination or electrophilic substitution reactions except under extreme conditions. Their improper disposal in storage and disposal area has negative impact on the ecosystem. Although Chemical methods are available for the degradation, they tend to emit more toxic chemicals. Alternative the biological methods are safer and cost effective. In this context, 2, 4 Dichlorobiphenyl, (a PCB congener) degrading bacterial isolates which have been evaluated for substrate affinity using PCB congener mix.

Methods: In the present study, 2, 3, 5, 6 tetrachlorobiphenyls and 3, 5’, 3, 5 tetrachlorobiphenyl in PCB congener mix degradation by previously characterised Pseudomonas isolates GSa and GSb was studied using GC-MS.

Findings: Two constantly overlapping bacterial isolates identified as Pseudomonas sp., capable of degrading 2, 4 Chlorobiphenyl degrading, showed its diversity of degrading other PCB congener mix. On GC-MS analysis of the cell free extract showed 60 and 70% degradation as per the ECD values.

Applications: Therefore, the present paper is first of its kind, as 2, 4 CB degrading Pseudomonads in tern degrade other PCB congeners perhaps showing wide application on in situ bioremediation since the contaminated site contains variety of congeners.


Keywords

Bioremediation, 2, 4 CB, PCB Congener, Pseudomonas sp, Diversity.
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  • UNEP, Guidelines for the identification of PCBs and materials containing PCBs. http://www.pops.int/documents/guidance/NIPsFinal/pcbid1.pdf . Date accessed: 1999.

  • C. Backe, I. T. Cousins, P. Larrson. PCB in soils and estimated soil air exchange fluxes of selected PCB congeners in the south of Sweden. Environmental Pollution, 2004; 128(1-2), 59-72.

  • C, Graham, J. J. Ramsden, Introduction to global warming, Complexity and security. IOS Press, 147-184. 2008.

  • M. D. Erickson, R. G. Kaley, Applications of Polychlorinated biphenyls, Environmental Science Pollution Research. 2011; 18, 135-151.

  • WHO, Environmental Health Criteria 140: Polychlorinated biphenyls and terphenyls. 2nd (Edn), Geneva. 1993.

  • C. Obinna, Nwinyi, Degradation of Askarel (PCB Blend) by indigenous aerobic bacteria isolates from dump sites in ore, Ondo State. Nigeria. Australian Journal of Basic and Applied Sciences. 2010;4,3938-3948.

  • D. H. Pieper, M, Seeger Bacterial metabolism of Polychlorinated biphenyls, Journal of Molecular Microbiology and Biotechnology. 2008; 15, 121-138.

  • D. H. Pieper, Aerobic degradation of Polychlorinated biphenyls. Applied Journal of Microbiology and Biotechnology. 2005; 67, 170-191.

  • R. Loch-Caruso. Uterine muscle as a potential target of Polychlorinated biphenyls during pregnancy. International Journal of Hygiene and Environmental Health. 2002; 205, 21-130.

  • S. Safe, K. Connor, K. Ramamoorthy, K. Gaido, S. Maness. Human exposure to endocrine active chemicals: Hazard assessment problems. Regulatory Toxicology and Pharmacology. 1997; 26; 52-58.

  • S. T. Vater, S. F. Valazquez, V. J. Cogliano. A case study of cancer data set combinations for PCBs. Regulatory Toxicology and Pharmacology. 1995; 22(1), 2-10.

  • De S. Flora, M. Bagnasco, P. Zanacchi. Genotoxic, carcinogenic and teratogenic hazards inthe marine environment with special references to the Mediterranean Sea. Mutation Research. 1991; 258, 285-320.

  • S. L. Schantz, J. J. Widholm, D. C. Rice. Effects of Polychlorinated biphenyls exposure on neuro psychological function in children. Environmental Health Perspectives. 2003; 111, 357-376.

  • G. Ross. The public health implications of Polychlorinated biphenyls (PCBs) in the environment, Ecotoxicology and Environmental Safety, 2004; 59, 275-291.

  • C. L. Portigal, S. P. Cowell, M. N. Fedorek, C. M. Butler, P. S. Renni, C. C. Nelson. Polychlorinated biphenyls interference with androgen–induced transcriptional activation and hormone binding. Toxicology and Applied Pharmacology. 2002; 179(3), 185-194.

  • G. Winneke, J. Walkowiak, H. Lilienthal. Polychlorinated biphenyl induced neuro developmental toxicity in human infants and its potential mediation by endocrine dysfunction. Toxicology. 2002; 181-182, 161-165.

  • O. M. Faroon, S. Keith, D. Jones, D. C. Rosa. Carcinogenic effects of Polychlorinated Biphenyls. Toxicology and Industrial Health. 2001; 17, 41-62.

  • Laura J. Amend, Peter B. Lederman. Critical evaluation of PCB remediation technologies. Environmental Progress and Sustainable energy. 1992; 11, 173-177.

  • W. R. Abraham, B. Nogales, P. N. Golyshin, D. H. Pieper, K. N. Timmis. Polychlorinated Biphenyl degrading microbial communities in soils and sediments. Current Opinion in Microbiology. 2002; 5, 246-253.

  • M. H. Hamdan , M. A. Othaman, Z. Suhaili, M. S. Shukor , M. I. E. Halmi , S. R. S. Abdullah, N. A. Shamaan, M. Y. Shukor. Isolation and characterization of a heavy metal reducing Enterobacteriaceae Bacterium Strain DRY 7 with the ability to assimilate phenol and diesel. Indian Journal of Science and Technology. 2016;9(23),1-10.

  • M. Seto, E. Masai, M. Ida, T. Hatta, M. Fukuda, K. Yano. Multi polychlorinated biphenyl transformation systems in the gram-positive bacterium Rhodococcus sp. strain RHA1. Applies and Environmental Microbiology. 1995; 61, 4510-4513.

  • M. Seeger, B. Cámara, B. Hofer. Dehalogenation, denitration, dehydroxylation, and angular attack on substituted biphenyls and related compounds by a biphenyl dioxygenase. Journal of Bacteriology. 2001; 183, 3548-3555.

  • M. Seeger, D. Pieper. Genetics of biphenyl degradation and co-metabolism of PCBs. In: Microbiology of hydrocarbons, oils, lipids and derived compounds. Timmis KN Edn., Springer, Heidelberg. 2009; 1179-1199.

  • J. D. Haddock, J. R. Horton, D. T. Gibson. Dihydroxylation and dechlorination of chlorinated biphenyls by purified biphenyl 2, 3 dioxygenase from Pseudomonas sp. strain LB400. Journal of Bacteriology. 1995; 177, 20-26.

  • Michael P. McLeod,René L. Warren, William W. L. Hsiao, Naoto Araki, Matthew Myhre, Clinton Fernandes, Daisuke Miyazawa, Wendy Wong, Anita L. Lillquist, Dennis Wang, Manisha Dosanjh, Hirofumi Hara, Anca Petrescu, Ryan D. Morin, George Yang, Jeff M. Stott, Jacqueline E. Schein, Heesun Shin, Duane Smailus, Asim S. Siddiqui, Marco A. Marra, Steven J. M. Jones, Robert Holt, Fiona S. L. Brinkman, Keisuke Miyauchi, Masao Fukuda, Julian E. Davies, William W. Mohn, Lindsay D. Eltis. The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proceedings of National Academy of Sciences USA 2006; 103(42), 15582-15587.

  • M. Sakai, S. Ezaki, N. Suzuki, R. Kurane. Isolation and characterization of a novel Polychlorinated-biphenyl degrading bacterium Paenibacillus sp. KBC 101. Journal of Applied Microbiology and Biotechnology. 2005; 68, 111-116.

  • K. J. Shobha, D. Gayathri. Degradation of 2, 4 dichlorobiphenyl via meta cleavage pathway by Pseudomonas spp. Consortium. Current Microbiology. 2015; 70, 871-876.

  • D. Gayathri, K. J. Shobha. 2,4 Dichlorobiphenyl, a congener of polychlorinated biphenyl degradation by Pseudomonas sp GSa and GSb. Indian Journal of Experimental Biology. 2015; 53, 536-542.

  • K. J. Shobha, D. Gayathri. Optimization of 2, 4 dichlorobiphenyl (PCB Congener) degradation by Pseudomonas spp. Bulletin of Environment and Scientific Research, 2014;3, 1-7.

  • J. G. Ibanez, H. Esparza, M. Doria, C. Serrano, F. Intante, M. M. Singh. Introduction to Environmental chemistry, Environmental Chemistry. 2007; XVII, 3-10.

  • P. K. Somaraja, D.Gayathri, N.Ramaiah. Molecular characterization of 2-Chlorobiphenyl degrading Stenotrophomonas maltophilia GS-103. Bulletin of Environmental Contamination and Toxicology, 2013; 91, 148-153.

  • Sunday A. Adebusoye, Flynn W. Picardal, Matthew O, Ilori O Olukayode. Amund Clay Fuqua. Characterization of multiple novel aerobic Polychlorinated biphenyl (PCB) utilizing bacterial strains indigenous to contaminated tropical African soils. Biodegradation, 2008; 19, 145-159.


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  • Diversity of 2, 4 Dichlorobiphenyl Degrading Consortium of Pseudomonas Isolates GSa and GSb for Degradation of Poly Chlorinated Biphenyl Congeners

Abstract Views: 432  |  PDF Views: 156

Authors

Shobha K. Jayanna
Department of Microbiology, Davangere University, Shivagangothri, Davangere-577002, India
Devaraja Gayathri
Department of Microbiology, Davangere University, Shivagangothri, Davangere-577002, India
Somaraja Palegar Krishnappa
Department of Microbiology, Davangere University, Shivagangothri, Davangere-577002, India

Abstract


Objective: Polychlorinated biphenyls (PCB) are persistent organic pollutants that are widely distributed in the environment. PCBs are aromatic compounds have more than 210 congeners, nonvolatile, chemically inert and do not undergo oxidation, reduction or addition reactions, elimination or electrophilic substitution reactions except under extreme conditions. Their improper disposal in storage and disposal area has negative impact on the ecosystem. Although Chemical methods are available for the degradation, they tend to emit more toxic chemicals. Alternative the biological methods are safer and cost effective. In this context, 2, 4 Dichlorobiphenyl, (a PCB congener) degrading bacterial isolates which have been evaluated for substrate affinity using PCB congener mix.

Methods: In the present study, 2, 3, 5, 6 tetrachlorobiphenyls and 3, 5’, 3, 5 tetrachlorobiphenyl in PCB congener mix degradation by previously characterised Pseudomonas isolates GSa and GSb was studied using GC-MS.

Findings: Two constantly overlapping bacterial isolates identified as Pseudomonas sp., capable of degrading 2, 4 Chlorobiphenyl degrading, showed its diversity of degrading other PCB congener mix. On GC-MS analysis of the cell free extract showed 60 and 70% degradation as per the ECD values.

Applications: Therefore, the present paper is first of its kind, as 2, 4 CB degrading Pseudomonads in tern degrade other PCB congeners perhaps showing wide application on in situ bioremediation since the contaminated site contains variety of congeners.


Keywords


Bioremediation, 2, 4 CB, PCB Congener, Pseudomonas sp, Diversity.

References