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Serum Enzyme and Histological Studies of Albino Rat Treated with Ethanol/potash Extract of Sorghum bicolor Leaf Sheath


Affiliations
1 Department of Chemistry, Rivers State University of Science and Technology, Port Harcourt, Nigeria
2 Department of Biochemistry, University of Port Harcourt, Choba, Nigeria
 

The effect of ethanol/potash extract of the leaf sheath of Sorghum bicolor on the histology and serum enzymes was investigated using wistar albino rat placed in four groups of five rats each. The following quantities: 1000, 2000 and 3000mg of the extract was administered orally to the albino rats per kg body weight, while 0.5ml of 0.9% normal saline solution served as control to the fourth group. The rats were dissected after 14 days. Results for Alanine Transaminase (ALT), Aspartate Transaminase (AST) and Alkaline phosphatase (ALP) ranges as follows: 30-46 U/L, 07/137 U/L and 38-130U/L respectively. The high values of these liver enzymes (ALT, AST and ALP) relative to control values are indicative of severe intra hepatic cell damage. Histopathological examination also, revealed lesion or alteration in the morphological features of the liver and kidney in all the animals. This sample used in the management of anaemia has been observed in rats under the conditions of this study to be toxic to both the liver and the kidney.

Keywords

Sorghum bicolor, Ethanol/potash, Serum Enzyme, Histological, Albino Rat
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  • Serum Enzyme and Histological Studies of Albino Rat Treated with Ethanol/potash Extract of Sorghum bicolor Leaf Sheath

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Authors

C. U. Ogunka-Nnoka
Department of Chemistry, Rivers State University of Science and Technology, Port Harcourt, Nigeria
A. A. Uwakwe
Department of Biochemistry, University of Port Harcourt, Choba, Nigeria
N. C. Nwachoko
Department of Chemistry, Rivers State University of Science and Technology, Port Harcourt, Nigeria

Abstract


The effect of ethanol/potash extract of the leaf sheath of Sorghum bicolor on the histology and serum enzymes was investigated using wistar albino rat placed in four groups of five rats each. The following quantities: 1000, 2000 and 3000mg of the extract was administered orally to the albino rats per kg body weight, while 0.5ml of 0.9% normal saline solution served as control to the fourth group. The rats were dissected after 14 days. Results for Alanine Transaminase (ALT), Aspartate Transaminase (AST) and Alkaline phosphatase (ALP) ranges as follows: 30-46 U/L, 07/137 U/L and 38-130U/L respectively. The high values of these liver enzymes (ALT, AST and ALP) relative to control values are indicative of severe intra hepatic cell damage. Histopathological examination also, revealed lesion or alteration in the morphological features of the liver and kidney in all the animals. This sample used in the management of anaemia has been observed in rats under the conditions of this study to be toxic to both the liver and the kidney.

Keywords


Sorghum bicolor, Ethanol/potash, Serum Enzyme, Histological, Albino Rat

References