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Determination of Antioxidant Property of Andrographis paniculata
Antioxidant property of Andrographis paniculata is evaluated in this study by employing three methods viz. DPPH, Lipid Peroxidation and DNA cleavage protective assay. First two methods employed, gallic acid and α- tocopherol as standard antioxidant for comparing with the antioxidant property of Andrographis paniculata; while in the DNA cleavage protective assay pBS plasmid was used to check the protective activity of Andrographis paniculata. In the DPPH method the RSC (radical scavenging capacity) of the plant extract was measured spectrophotometrically at 512nm. The lipid peroxidation inhibition activity of plant extract was also evaluated by taking the absorbance of pink color complexed formed at 535nm. In the DNA cleavage protective assay, the electrophoretic pattern of DNA after UV-induced photolysed H2O2 - oxidative damage showed difference in the absence and presence of methanolic extracts of the plant. This study indicates the methanolic extracted of the Andrographis paniculata has higher antioxidant activity than water and methanolic extract.
Keywords
Antioxidant Property, Andrographis paniculata, Free Radical, Methanolic Extract
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- Birnboim HC and Doly JA (1979) Rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 24, 1513-1523
- Blois MS (1975) Antioxidant determinations by the use of a stable free radical. Nature. 181,1199-2000.
- Buege JA and Aust SD (1978) Microsomal lipid peroxidation. Methods in Enzymology.52, 302-310.
- Chattopadhyay SK, Datta SK and Mahato SB (1994) Plant Cell Report.13, 519-522.
- D'Mello PM, Jadhav MA and Jolly CI (2000) Indian Drugs. 37,518.
- Ish-Horowicz D and Burke JF (1981) Rapid and efficient cosmid cloning. Nucleic Acid Res. 9, 2989- 2998.
- Jarukamjorn K and Nemoto N (2008) Pharmacological aspects of Andrographis paniculata on health and its magor diterpenoid constituent andrographolide. J. Health Sci. 54,370-381.
- Kapoor LD (2001) Handbook of Ayurvedic Medicinal Plants. Crc Press, Florida, 416.
- Kebapci N, Belgin EF, Fahrettin A, Emine S and Canan D (1999) Oxidative stress and antioxidant therapy in Type -II diabetes mellitus. Turk. JEM. 4, 153-162.
- Kiritikar KR and Basu BD (1975) Indian Medicinal Plants, Periodical experts book agency, Delhi. 3,1965.
- Kuyvenhoven JP and Meinders AE (1999) Oxidative stress and diabetes mellitus Pathogenesis of longterm complications. Eur. J. Intern. Med., 10, 9-19.
- Niki E, Shimaski H and Mino M (1994) Antioxidation-free radical and biological defence. Gakkai Syuppn Center, Tokyo, pp:3-16.
- Osawa T (1990) Antimutagenesis and anticarcinogenesis mechanism, New York: Plenum.pp:139.
- Puri A, Saxena R, Saxena, RP, Saxena, KC, Srivastava, V and Tandon JS (1993) Immunostimulant agents from Andrographis paniculata. J. Nat. Prod., 56: 995- 999.
- Ramarathnam N, Ochi H and Takeuchi M (1997) Antioxidative Defence System in Vegetables Extracts. In: Natural antioxidants, chemistry, health effects, and applications. Shahidi F (ed.), AOCS Press, Champaign, Illinois, pp: 76-87.
- Russo A, Izzo AA, Cardile V, Borrelli F and Vanella A (2001) Indian medicinal plants as antiradicals and DNA cleavage protectors. Phytomedicine, 8, 125-132.
- Shahidi F (1997) Natural antioxidant: an overview, n: Natural antioxidants, chemistry, health effects, and applications. Shahidi F (ed.), AOCS Press, Champaign, Illinois, pp: 1-11.
- Wang DW and Zhao HY (1994) Prevention ofatherosclerotic arterial stenosis and restenosis after angioplasty with Andrographis paniculata nees and fish oil. Clin. Med.J .Engl.107, 464-470.
- Zhang XF and Tan BK (2000) Antihyperglycemic and antioxidant properties of Andrographis paniculata in normal and diabetic rats. Clin. Exp. Pharmacol. Physiol. 27, 358-363.
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