Determination of Antioxidant Property of Andrographis paniculata

Antioxidant property of Andrographis paniculata is evaluated in this study by employing three methods viz. DPPH, Lipid Peroxidation and DNA cleavage protective assay. First two methods employed, gallic acid and α- tocopherol as standard antioxidant for comparing with the antioxidant property of Andrographis paniculata; while in the DNA cleavage protective assay pBS plasmid was used to check the protective activity of Andrographis paniculata. In the DPPH method the RSC (radical scavenging capacity) of the plant extract was measured spectrophotometrically at 512nm. The lipid peroxidation inhibition activity of plant extract was also evaluated by taking the absorbance of pink color complexed formed at 535nm. In the DNA cleavage protective assay, the electrophoretic pattern of DNA after UV-induced photolysed H₂O₂ - oxidative damage showed difference in the absence and presence of methanolic extracts of the plant. This study indicates the methanolic extracted of the Andrographis paniculata has higher antioxidant activity than water and methanolic extract.