Investigating Effects of Salvia Officinalis (Sage) on Development of Mice Embryos Kidney and Some Hormonal Effect of Treated Mothers

Background Salvia officinalis (sage) is commonly employed in both medicinal and culinary preparations, Salvia plants are antioxidant and can improve ‘head and brain’ function. Aim of study: To examine effects of aqueous plant extract of sage on development of mouse embryos kidney and hormonal effect of treated mothers. Material and Method: Thirty adult albino Swiss female mice were used in this experiments study, their weight was 23-27 gm, three groups with 10 mice per group were categorized as: G(A) orally treated with sage aqueous extract (167.8) mg/kg, G(B) administration orally with sage aqueous extract (83.9) mg/kg, G(C) orally treated with distilled water (control group) for 6 weeks. For histological study, the pregnant female mice have been sacrificed at day 17 of gestation of each group and 30 mice embryos were fixed in Bouin’s fixative, paraffin infiltration. Specimen stains with H&E. After 24 hours from last orally dose of treatment period, 3 animals from each groups were sacrificed under ether anesthesia, 3 ml blood sample was collected from each animals, serum reproductive hormonal assay of FSH, LH, E2 and P4 were performed depends on kit assay procedure of ELISA kit. Result :Histological examination of embryonic kidney after maternally oral administrated of sage reveals, different histopathological changes of embryonic renal tissue, also results revealed an increase that is highly significant (P< 0.01) in the numbers of fetuses belongs to G(A) and G(B) mothers at day 17 in comparison with G(C). Statistical analysis revealed in contrast with G(C) a highly significant (P< 0.01) rise in weights of fetuses (males and females) belonged to mothers of G(A) and G(B), while, serum hormones level show highly significant (p≤0.01) rise in E2 and P4 level in G(B) when compared with G(A), while significant decrease (P<0.05) in FSH level in G(A) and G(B), and there was a significant rise (P<0.05) in LH hormone level in G(A) and G(B).