Indian Journal of Forensic Medicine & Toxicology

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In-Vitro Cytotoxic Study of Moullava Spicata (Dalz.) Nicolson Leaf Extract


Affiliations
1 Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri
2 Central Research Laboratory, A B Shetty Memorial Institute of Dental Sciences, Nitte University, India
3 Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri, India
     

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Objectives: The aim of present study is to evaluate cytotoxic and genotoxic activity of Moullava spicata (Dalz.) Nicolson.

Materials and Method: The aqueous extract of this plant subjected to genotoxic and cytotoxic study using yeast viability test, hemolytic assay and Allium test.

Result: The extract showed dose dependent effect on yeast viability and hemolysis of erythrocytes. The Allium test showed significant reduction of ischolar_main length (p > 0.01) and number (p > 0.001) indicated cytotoxic effect of plant extract. Microscopic observations showed cytotoxic effects such as ghost cell, membrane damage or rupture, apoptotic bodies and the extent damage was increased significantly after 96 hours treatment.

Conclusion: The study showed that the leaf extract of the M. spicata contains various biologically active compounds and possess cytotoxic effects.


Keywords

Moullava spicata (Dalz.) Nicolson, Allium test, Hemolysis, Cytotoxicity
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  • In-Vitro Cytotoxic Study of Moullava Spicata (Dalz.) Nicolson Leaf Extract

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Authors

K. Lohith
Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri
R. Vijay
Central Research Laboratory, A B Shetty Memorial Institute of Dental Sciences, Nitte University, India
K. C. Pushpalatha
Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri, India
Chandrashekar G. Joshi
Department of Biochemistry, Mangalore University, P.G Centre, Cauvery Campus, Madikeri, India

Abstract


Objectives: The aim of present study is to evaluate cytotoxic and genotoxic activity of Moullava spicata (Dalz.) Nicolson.

Materials and Method: The aqueous extract of this plant subjected to genotoxic and cytotoxic study using yeast viability test, hemolytic assay and Allium test.

Result: The extract showed dose dependent effect on yeast viability and hemolysis of erythrocytes. The Allium test showed significant reduction of ischolar_main length (p > 0.01) and number (p > 0.001) indicated cytotoxic effect of plant extract. Microscopic observations showed cytotoxic effects such as ghost cell, membrane damage or rupture, apoptotic bodies and the extent damage was increased significantly after 96 hours treatment.

Conclusion: The study showed that the leaf extract of the M. spicata contains various biologically active compounds and possess cytotoxic effects.


Keywords


Moullava spicata (Dalz.) Nicolson, Allium test, Hemolysis, Cytotoxicity

References