Indian Journal of Forensic Medicine & Toxicology

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Restriction Fragment Length Polymorphism (RFLP) Application in DNA Typing for Crime Investigation


Affiliations
1 Department of Biochemistry, College of Medicine, Al-Jouf University 2014, Sakaka, Al-Jouf, Kingdom of Saudi Arabia, Saudi Arabia
2 Department of Biotechnology & Molecular Biology, BioAxis DNA Research Centre, Hyderabad 500 037, India
     

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DNA typing is on the rise in its use as an identification aid for forensic scientists. DNA typing have opened a new frontier in forensic analysis. Until 1985 all polymorphic immunological and biochemical markers employed to identify biological samples in criminal cases had limited applicability. The way to a new course of events was first paved by the introduction of minisatellites using multilocus probes (MLPs), providing incomparably higher discriminatory power. However, the procedures for multilocus typing were too arduous and required very large amounts of intact DNA which were usually not recovered from the difficult forensic biological specimens. This paved way for initiation of single locus probes (SLPs) in VNTR (variable number of tandem repeats) detection analysis. Analysis was stared with isolation of DNA from samples done by chloroform: isoamylalcohol method followed by the estimation of DNA in the samples with a standard DPA (method by comparing with standard commercial DNA. After that RFLP method was followed. The aim of our paper is identification of crime assailant with the aid of current DNA technology.

Keywords

DNA Typing, RFLP, Forensic Investigation, VNTR, DPA, Minisatellites
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  • Restriction Fragment Length Polymorphism (RFLP) Application in DNA Typing for Crime Investigation

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Authors

Shaik. Rahiman
Department of Biochemistry, College of Medicine, Al-Jouf University 2014, Sakaka, Al-Jouf, Kingdom of Saudi Arabia, Saudi Arabia
Praveena Nissankararao
Department of Biotechnology & Molecular Biology, BioAxis DNA Research Centre, Hyderabad 500 037, India
M. Phani Kumar
Department of Biotechnology & Molecular Biology, BioAxis DNA Research Centre, Hyderabad 500 037, India

Abstract


DNA typing is on the rise in its use as an identification aid for forensic scientists. DNA typing have opened a new frontier in forensic analysis. Until 1985 all polymorphic immunological and biochemical markers employed to identify biological samples in criminal cases had limited applicability. The way to a new course of events was first paved by the introduction of minisatellites using multilocus probes (MLPs), providing incomparably higher discriminatory power. However, the procedures for multilocus typing were too arduous and required very large amounts of intact DNA which were usually not recovered from the difficult forensic biological specimens. This paved way for initiation of single locus probes (SLPs) in VNTR (variable number of tandem repeats) detection analysis. Analysis was stared with isolation of DNA from samples done by chloroform: isoamylalcohol method followed by the estimation of DNA in the samples with a standard DPA (method by comparing with standard commercial DNA. After that RFLP method was followed. The aim of our paper is identification of crime assailant with the aid of current DNA technology.

Keywords


DNA Typing, RFLP, Forensic Investigation, VNTR, DPA, Minisatellites

References