A Study of Free Radical Changes in Edb (Ethylene Dibromide) Toxicity

Effect of Ethylene dibromide (EDB) administration on antioxidant enzymes in rabbit liver was studied. The animals were given experimental diets 0.075 ml EDB/rabbit along with alcohol. In EDB group 24 hr. Urine chemistry and blood samples were examined heart and liver sections were stained with Von kossa's stain for histological examination.

Antioxidant enzymes and lipid peroxidation were measured in tissues and Blood samples. Results revealed significantly increased levels of Urine creatinine (Pre 36.40 ± 4.12; post 36.66 ± 3.92 mg/dl) Urea (Pre -649.20 ± 73.72; post 670.83 ± 6292 mg/dl) serum GPT (Pre 10.41 ± 1.48; Post -19.75 ± 0.95 lo/L) in rabbit blood after EDB administration.

Plasma ascorbic acid (Pre 1.00 ± 0.18; Post 0.87 ± 0.19 mg/dl), erythrocyte SOD (Pre 2.47 ± 0.33; Post 1.86 ± 0.22 Units/ml Haemolysate), GSH-Px (Pre 7.14 ± 1.16; Post 5.23 ± 1.12 mg GSH/min at 37°C) and GSH (Pre -58.62 ± 14.55; Post -1.86 ± 0.22 mg/dl) levels decreased as SOD activity increases. Increased TBARS production after EDB treatment (Pre 2.66 ± 0.99; Post 4.00 ± 0.82 nmol/ml) in the liver indicate that the organ is being subjected to oxidative stress in acute EDB poisoning.