Indian Journal of Geo-Marine Sciences

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Bacterial and archaeal communities in near-surface marine sediments from İzmir Bay (Eastern Aegean Sea), using Quantitative PCR


Affiliations
1 Dokuz Eylul University, Institute of Marine Sciences and Technology, Inciralti-İzmir – 35340,, Turkey
2 Dokuz Eylul University, The Graduate School of Natural and Applied Sciences, İzmir – 35390,, Turkey

Marine sediments are good sources of both subsistent and anthropogenic ingredients. Recently, an assessment of phylogenetic analysis at the genotypic and molecular levels has revealed the phylogenetic relationships of the prokaryotes. In our study, samples were collected from the surface of sediments in the İzmir Bay, located in the eastern Aegean Sea and surrounded by the coastal city of İzmir. Total genomic DNA was extracted from 0.25 g sediment of each sample and used in real-time qPCR to detect 16S rDNA genes of archaea, bacteria, sulfate-reducing bacteria, Desulfobulbus, and Desulfosarcina-Desulfococcus. Our results showed that the maximum number of all samples was found in bacterial primers (1.24×109 DNA copy numbers/g), while the minimum number was observed in Desulfosarcina-Desulfococcus primers (1.17×102 DNA copy numbers/g). Consequently, genetic studies should focus on investigating the abundance levels of seasonal and annual microbial groups in future studies.
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  • Bacterial and archaeal communities in near-surface marine sediments from İzmir Bay (Eastern Aegean Sea), using Quantitative PCR

Abstract Views: 164  | 

Authors

A Kacar
Dokuz Eylul University, Institute of Marine Sciences and Technology, Inciralti-İzmir – 35340,, Turkey
B Omuzbuken
Dokuz Eylul University, The Graduate School of Natural and Applied Sciences, İzmir – 35390,, Turkey

Abstract


Marine sediments are good sources of both subsistent and anthropogenic ingredients. Recently, an assessment of phylogenetic analysis at the genotypic and molecular levels has revealed the phylogenetic relationships of the prokaryotes. In our study, samples were collected from the surface of sediments in the İzmir Bay, located in the eastern Aegean Sea and surrounded by the coastal city of İzmir. Total genomic DNA was extracted from 0.25 g sediment of each sample and used in real-time qPCR to detect 16S rDNA genes of archaea, bacteria, sulfate-reducing bacteria, Desulfobulbus, and Desulfosarcina-Desulfococcus. Our results showed that the maximum number of all samples was found in bacterial primers (1.24×109 DNA copy numbers/g), while the minimum number was observed in Desulfosarcina-Desulfococcus primers (1.17×102 DNA copy numbers/g). Consequently, genetic studies should focus on investigating the abundance levels of seasonal and annual microbial groups in future studies.