Indian Journal of Geo-Marine Sciences

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In-Vitro Screening of Amylase Producing Halophilic Bacteria Isolated from Seawater


Affiliations
1 Department of Microbiology, Kamaraj College of Arts and Science, Thoothukudi, Tamil Nadu – 628 003, India
2 School of Bioscience, Faculty of Medicine, Bioscience & Nursing, Jalan SP2, Bandar Saujana Putra 42610 Jenjarom, Selangor, Malaysia
3 Department of Biotechnology, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu – 600 119, India
4 Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu – 632 014, India
 

In this study, eight halobacterial strains were isolated from seawater. Among the eight isolates, four strains grew well in nutrient medium with 40 g/100 mL of sodium chloride concentration. Amongst all, HA01 was found to show highest amylase activity than others. Highest amylase producing bacteria, i.e. HA01 was subjected for 16S rRNA sequence based identification and it was found to be Aquabacterium sp. strain AS02. The organism showed optimal amylase production at pH 7 and temperature of 42 °C and NaCl concentration of 3 M. The two substrates (banana peel and potato peel) were used for the amylase production among which banana peel showed that highest amylase activity of 6.12 U/mL than potato peel (4.27 U/mL).

Keywords

Amylase, Halophilic Bacteria, Optimization.
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  • In-Vitro Screening of Amylase Producing Halophilic Bacteria Isolated from Seawater

Abstract Views: 183  |  PDF Views: 98

Authors

S. Abirami
Department of Microbiology, Kamaraj College of Arts and Science, Thoothukudi, Tamil Nadu – 628 003, India
M. Muthu Lakshmi
Department of Microbiology, Kamaraj College of Arts and Science, Thoothukudi, Tamil Nadu – 628 003, India
A. V. Samrot
School of Bioscience, Faculty of Medicine, Bioscience & Nursing, Jalan SP2, Bandar Saujana Putra 42610 Jenjarom, Selangor, Malaysia
P. J. Jane Cypriyana
Department of Biotechnology, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu – 600 119, India
S. Saigeetha
Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu – 632 014, India
G. Narendrakumar
Department of Biotechnology, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu – 600 119, India

Abstract


In this study, eight halobacterial strains were isolated from seawater. Among the eight isolates, four strains grew well in nutrient medium with 40 g/100 mL of sodium chloride concentration. Amongst all, HA01 was found to show highest amylase activity than others. Highest amylase producing bacteria, i.e. HA01 was subjected for 16S rRNA sequence based identification and it was found to be Aquabacterium sp. strain AS02. The organism showed optimal amylase production at pH 7 and temperature of 42 °C and NaCl concentration of 3 M. The two substrates (banana peel and potato peel) were used for the amylase production among which banana peel showed that highest amylase activity of 6.12 U/mL than potato peel (4.27 U/mL).

Keywords


Amylase, Halophilic Bacteria, Optimization.

References