Indian Journal of Geo-Marine Sciences

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Enhancement of Bacteriorhodopsin Production from Novel Haloarchaea Strains of Marakanam Region of Tamil Nadu


Affiliations
1 Centre for Ocean Research, Sathyabama Institute of Science and Technology, Chennai – 600 119, India
2 Marine Biotechnology, National Institute of Ocean Technology, Chennai – 600 119, India
 

Haloarchaea from high saline habitats are known to contain industrially important value-added bioactive compounds such as bacteriorhodopsin, carotenoids, lipids, and proteins. The diversity of Haloarchaea from geographically less explored saline habitats has received less attention. In this study isolation and characterization of the biotechnological potential of novel haloarchaea strains from solar saltern, a hypersaline environment in Marakanam, Tamilnadu was carried out. The samples were collected during the pre-monsoon (July) and post-monsoon (January) season and ten different haloarchaea strains were isolated. UV-Vis spectroscopy analysis revealed the bacteriorhodopsin production by all the isolated strains. Further experiments were carried out to estimate the yield of bacteriorhodopsin and to assess their photocurrent activity and biosensor applications. The maximum yield of bacteriorhodopsin was 5.6 mg/L of cultured lysate. This study will contribute to a better understanding on effect of elicitors on bacteriorhodopsin producing different haloarchaea strains and growing knowledge on their biotechnological applications.

Keywords

Bacteriorhodopsin, Elicitors, Haloarchaea, Purple Membrane, Salt Pan.
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  • Soppa J, From genomes to function: haloarchaea as model organisms, Microbiology, 152 (3) (2006) 585-590.

  • Henderson R, The structure of the purple membrane from Halobacterium halobium: analysis of the X-ray diffraction pattern, H Mol Biol, 93 (2) (1975) 123-138.

  • Danon A & Stoeckenius W, Photophosphorylation in Halobacterium halobium, Proc Natl Acad Sci, 71 (4) (1974) 1234-1238.

  • Koch M K & Oesterhelt D, MpcT is the transducer for membrane potential changes in Halobacterium salinarum, Mol Microbiol, 55 (6) (2005) 1681-1694.

  • Seyedkarimi M S, Aramvash A & Ramezani R, High production of bacteriorhodopsin from wild type Halobacterium salinarum, Extremophiles, 19 (5) (2015) 1021-1028.

  • Puthenveetil R & Vinogradova O, Optimization of the design and preparation of nanoscale phospholipid bilayers for its application to solution NMR, Protein, 81 (7) (2013) 1222-1231.

  • Luque R, González-Domenech C M, Llamas I, Quesada E & Béjar V, Diversity of culturable halophilic archaea isolated from Rambla Salada, Murcia (Spain), Extremophiles, 16 (2) (2012) 205-213.

  • Hampp N, Bacteriorhodopsin as a photochromic retinal protein for optical memories, Chem Rev, 100 (5) (2000) 1755-1776.

  • Lee S Y, Chang H N, Um Y S & Hong S H, Bacteriorhodopsin production by cell recycle culture of Halobacterium halobium, Biotechnol Lett, 8 (1998) 763–765.

  • Hampp N & Oesterhelt D, Bacteriorhodopsin and its potential in technical applications, In: Nanobiotechnology: concepts, applications and perspectives, edited by Niemeyer C M & Mirkin C A, (Wiley, Weinheim), 2004.

  • Kalenov S V, Baurina M M, Skladnev D A & Kuznetsov A Y, High-effective cultivation of Halobacterium salinarum providing with bacteriorhodopsin production under controlled stress, J Biotechnol, 233 (2016) 211-218.

  • Manikandan M, Pasi C L & Kannan V, Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology, Bioresour Technol, 100 (12) (2009) 3107–3112.

  • Shiu P J, Chen H M & Lee C K, One-step purification of delipidated Bacteriorhodopsin by aqueous-three-phase system from purple membrane of Halobacterium, Food Bioprod Process, 92 (2) (2014) 113–119.

  • Kahaki F A, Babaeipour V, Rajabi H & Mofid M R, High overexpression and purification of optimized bacterio-opsin from Halobacterium salinarum R1 in E. coli, Appl Biochem Biotechnol, 174 (4) (2014) 1558–1571.

  • Lu H, Yuan W, Zhou J & Chong P L G, Glucose synthesis in a protein-based artificial photosynthesis system, Appl Biotechnol Biochem, 177 (1) (2015) 105-117.

  • Peck R F, brp and blh are required for synthesis of the retinal cofactor of bacteriorhodopsin in Halobacterium salinarum, J Biol Chem, 276 (2001) 5739–5744.

  • Ispirli N H, Gulluce M, Karadayi M & Demir A Y, Culturable bacteriorhodopsin-producing haloarchaea of Tuz Lake (Turkey), Geomicrobiol J, 36 (9) (2019) 831-836.

  • Shand R F & Betlach M C, Expression of the bop gene cluster of Halobacterium halobium is induced by low oxygen tension and by light, J Bacteriol, 173 (1991) 4692–4699.

  • Beard S J, Hayes P K & Walsby A E, Growth competition between Halobacterium salinarum strain PHH1 and mutants affected in gas vesicle synthesis, Microbiol, 143 (2) (1997) 467-473.

  • Dussault H P, An improved technique for staining red halophilic bacteria, J Bacterial, 70 (4) (1955) 484-485.

  • Spudich J L & Jung K H, Microbial rhodopsins: phylogenetic and functional diversity, Handbook of photosensory receptors, (2005) 1-23.

  • Boujelben I, Gomariz M, Martínez-García M, Santos F, Peña A, et al., Spatial and seasonal prokaryotic community dynamics in ponds of increasing salinity of Sfax solar saltern in Tunisia, Antonie Van Leeuwenhoek, 101 (4) (2012) 845-857.

  • Gasol J M, Casamayor E O, Joint I, Garde K, Gustavson K, et al., Control of heterotrophic prokaryotic abundance and growth rate in hypersaline planktonic environments, Aquat Microbial Ecol, 34 (2) (2004) 193-206.

  • Podell S, Emerson J B, Jones C M, Ugalde J A, Welch S, et al., Seasonal fluctuations in ionic concentrations drive microbial succession in a hypersaline lake community, ISME J, 8 (5) (2014) 979-990.

  • Ram-Mohan N, Oren A & Papke R T, Analysis of the bacteriorhodopsin-producing haloarchaea reveals a core community that is stable over time in the salt crystallizers of Eilat, Israel, Extremophiles, 20 (5) (2016) 747-757.


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  • Enhancement of Bacteriorhodopsin Production from Novel Haloarchaea Strains of Marakanam Region of Tamil Nadu

Abstract Views: 136  |  PDF Views: 86

Authors

J. Steffy
Centre for Ocean Research, Sathyabama Institute of Science and Technology, Chennai – 600 119, India
L. S. Abraham
Centre for Ocean Research, Sathyabama Institute of Science and Technology, Chennai – 600 119, India
D. Inbakandan
Centre for Ocean Research, Sathyabama Institute of Science and Technology, Chennai – 600 119, India
K. Govindaraju
Centre for Ocean Research, Sathyabama Institute of Science and Technology, Chennai – 600 119, India
G. Dharani
Marine Biotechnology, National Institute of Ocean Technology, Chennai – 600 119, India

Abstract


Haloarchaea from high saline habitats are known to contain industrially important value-added bioactive compounds such as bacteriorhodopsin, carotenoids, lipids, and proteins. The diversity of Haloarchaea from geographically less explored saline habitats has received less attention. In this study isolation and characterization of the biotechnological potential of novel haloarchaea strains from solar saltern, a hypersaline environment in Marakanam, Tamilnadu was carried out. The samples were collected during the pre-monsoon (July) and post-monsoon (January) season and ten different haloarchaea strains were isolated. UV-Vis spectroscopy analysis revealed the bacteriorhodopsin production by all the isolated strains. Further experiments were carried out to estimate the yield of bacteriorhodopsin and to assess their photocurrent activity and biosensor applications. The maximum yield of bacteriorhodopsin was 5.6 mg/L of cultured lysate. This study will contribute to a better understanding on effect of elicitors on bacteriorhodopsin producing different haloarchaea strains and growing knowledge on their biotechnological applications.

Keywords


Bacteriorhodopsin, Elicitors, Haloarchaea, Purple Membrane, Salt Pan.

References