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Evaluation of biochemical and antioxidant properties of crude extracts from the Indian cattle leech Poecilobdella granulosa


Affiliations
1 Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu – 600 025, India., India
 

Oxidative stress is one of the factors influencing the structure and function of cells, tissues, and their components. Natural antioxidants have emerged as prophylactic and therapeutic agents for diseases like cancer and cardiovascular disease. The objective of the current study is to assess the biochemical composition and antioxidant potential of crude extracts from various organs of the Indian cattle leech Poecilobdella granulosa. The biochemical constituents and protein profiles of various crude samples were determined by Native and SDS polyacrylamide gel electrophoresis. Moreover, four different crude samples were subjected to check free radical scavenging activity. In the biochemical analysis, protein and carbohydrate content were found to be higher in whole-body extract and several major and minor bands were found ranging from 22 to 110 kDa during qualitative analysis. Interestingly, among the four crude samples, saliva alone showed antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and superoxide anion radical scavenging activity. This study concluded that the crude saliva extract from P.granulosais a highly selective and effective antioxidant for human welfare.

Keywords

2, 2-Diphenyl-1-Picrylhydrazyl, Antioxidant, Free Radical, Poecilobdella Granulosa, Saliva, Superoxide.
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  • Evaluation of biochemical and antioxidant properties of crude extracts from the Indian cattle leech Poecilobdella granulosa

Abstract Views: 83  |  PDF Views: 59

Authors

D Padmanaban
Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu – 600 025, India., India
R G Muthusamy
Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu – 600 025, India., India
G J Sahayanathan
Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu – 600 025, India., India
K Raja
Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu – 600 025, India., India
A Chinnasamy
Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu – 600 025, India., India

Abstract


Oxidative stress is one of the factors influencing the structure and function of cells, tissues, and their components. Natural antioxidants have emerged as prophylactic and therapeutic agents for diseases like cancer and cardiovascular disease. The objective of the current study is to assess the biochemical composition and antioxidant potential of crude extracts from various organs of the Indian cattle leech Poecilobdella granulosa. The biochemical constituents and protein profiles of various crude samples were determined by Native and SDS polyacrylamide gel electrophoresis. Moreover, four different crude samples were subjected to check free radical scavenging activity. In the biochemical analysis, protein and carbohydrate content were found to be higher in whole-body extract and several major and minor bands were found ranging from 22 to 110 kDa during qualitative analysis. Interestingly, among the four crude samples, saliva alone showed antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and superoxide anion radical scavenging activity. This study concluded that the crude saliva extract from P.granulosais a highly selective and effective antioxidant for human welfare.

Keywords


2, 2-Diphenyl-1-Picrylhydrazyl, Antioxidant, Free Radical, Poecilobdella Granulosa, Saliva, Superoxide.

References