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Characterization and biomedical application of Tetradecamethylcycloheptasiloxane, a silicone-type biosurfactant produced by Streptomyces castaneoglobisporus AJ9 isolated from solar salt works


Affiliations
1 Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Tamilnadu – 629 502, India., India
 

Tetradecamethylcycloheptasiloxane (C14H42O7 Si7 ), a biosurfactant, was extracted by acid precipitation method from the haloalkaliphilic actinomycetes, Streptomyces castaneoglobisporus AJ9 (Gene Bank KC603900.1) and characterized by GC-MS analysis. The purified biosurfactant could effectively degrade dyes such as orange MR, direct violet, cotton red, reactive yellow and nitro green. Antimicrobial screening results showed that biosurfact ants could effectively control bacterial pathogens such as Staphylococcus aureus and Vibrio parahaemolyticus and fungal pathogens such as Fusarium sp. and Penicillium sp. Even a small diminutive quantity of biosurfactant (100 μg) could totally block the multiplication of White Spot Syndrome Virus (WSSV) in shrimp in vivo. The suppression of cancer cells in vitro by the biosurfactant was 74 % as confirmed by Response Surface Methodology (RSM) and residual plot analysis. Wheat bran, groundnut oilcake and oilseed cake seem to enhance biosurfactant production.

Keywords

Antimicrobials, Biosurfactants, Streptomyces castaneoglobisporus, Tetradecamethylcycloheptasiloxne.
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  • Rodrigues L R, Teixeira J A, van der Mei H C & Oliveira R, Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53, Colloids Surf B Biointerfaces, 49 (1) (2006) 79–86.
  • Margesin R & Schinner F, Bioremediation (natural attenuation and biostimulation) of diesel-oil contaminated soil in alpine glacier skiing area, Appl Environ Microbiol, 67 (7) (2001) 3127–3133.
  • Galinski E A & Tindall B J, Biotechnological prospects for halophiles and halotolerant microorganisms, In: Molecular biology and biotechnology of extremophiles, edited by Herbert R A & Sharp R J, (Blackie & Sons Ltd, Glasgow, UK), 1992, pp. 76–114.
  • Yin J, Chen J-C, Wu Q & Chen G-Q, Halophiles, coming stars for industrial biotechnology, Biotechnol Adv, 33 (7) (2015) 1443–1442.
  • Singh P & Cameotra S S, Potential application of microbial surfactants in biomedical sciences, Trends Biotechnol, 22 (3) (2004) 142–146.
  • Cameotra S S & Makkar R S, Recent applications of biosurfactants as biological and immunological molecules, Curr Opin Microbiol, 7 (3) (2004) 262–266.
  • Bhaskar P V & Bhosle N B, Microbial extracellular polymeric substances in marine biogeochemical processes, Curr Sci, 88 (1) (2005) 45–53.
  • Kosaric N, Biosurfactants in industry, Pure Appl Chem, 64 (11) (1992) 1731–1737.
  • Gudiña E J, Rangarajan V, Sen R & Rodrigues L R, Potential therapeutic applications of biosurfactants, Trends Pharmacol Sci, 34 (12) (2013) 667–675.
  • Harshada K, Biosurfactant: A potent antimicrobial agent, J Microbiol Exp, 1 (5) (2014) 173–177.
  • Sen R, Response surface optimization of the critical media components for the production of surfactin, J Chem Tech Biotechnol, 68 (3) (1997) 263–270.
  • Parekh V J & Pandit A B, Solid State Fermentation (SSF) for the production of sophorolipids from Starmerella bombicola NRRL Y-17069 using glucose, wheat bran and oleic acid, Curr Trends Biotechnol Pharma, 6 (4) (2012) 418–424.
  • Makkar R S & Cameotra S S, An update on the use of unconventional substrates for biosurfactant production and their new applications, Appl Microbial Biotechnol, 58 (4) (2002) 428–434.
  • Rodrigues L, Banat I M, Teixeira J & Oliveira R, Biosurfactants: potential application in medicine, J Antimicro Chemo, 57 (4) (2006) 609–618.
  • Maneerat S, Production of biosurfactants using substrates from renewable-resources, Songklanakarin J Sci Technol, 27 (3) (2005) 675–683.
  • Remya R, Screening and characterization of pharmacological important secondary metabolites from halophilic Streptomyces sp., M. Sc dissertation, Manonmaniam Sundaranar University, India (2012).
  • Remya R, Screening and characterization of biosurfactants from haloalkaliphilic actinomycetes isolated from solar salt works in Kanyakumari district, M. Phil Dissertation, Manonmaniam Sundaranar University, India (2013).
  • Jain D K, Collins-Thompson D L, Lee H & Trevors J T, A drop-collapsing test for screening surfactant-producing microorganisms, J Microbiol Methods, 13 (4) (1991) 271–279.
  • Youssef N H, Duncan K E, Nagle D P, Savage K N, Knapp R M, et al., Comparison of methods to detect biosurfactant production by diverse microorganisms, J Microbiol Methods, 56 (3) (2004) 339–347.
  • Morita T, Konishi M, Fukuoka T, Imura T & Kitamoto D, Microbial conversion of glycerol into glycolipid biosurfactants, mannosylerythritol lipids, by a basidiomycete yeast, Pseudozyma antarctica JCM 10317, J Biosci Bioeng, 104 (1) (2007) 78–81.
  • Cooper D G & Goldenberg B G, Surface-active agents from two Bacillus species, Appl Environ Microbiol, 53 (2) (1987) 224–229.
  • Kalme S D, Parshetti G K, Jadhav S U & Govindwar S P, Biodegradation of benzidine based dye Direct Blue-6 by Pseudomonas desmolyticum NCIM 2112, Bioresour Technol, 98 (7) (2007) 1405–1410.
  • Pruthi V & Cameotra S S, Production of a biosurfactant exhibiting excellent emulsification and surface-active properties by Serratia marcescens, World J Microbiol Biotechnol, 13 (1997) 133–135.
  • Bauer A W, Kirby W M, Sherris J C & Truck M, Antibiotic susceptibility testing by a standardized single disk method, Am J Clin Pathol, 45 (4) (1966) 493–496.
  • Balasubramanian G, Sarathi M, Rajesh Kumar S & Sahul Hameed A S, Screening the antiviral activity of Indian medicinal plants against white spot syndrome virus in shrimp, Aquaculture, 263 (1) (2006) 15–19.
  • Chang C F, Su M S, Chen H Y, Lo C F, Kou G H, et al., Effect of dietary beta-1.3-glucan on resistance to white spot syndrome virus (WSSV) in postlarval and juvenile Penaeus monodon, Dis Aquat Organ, 36 (1999) 163–168.
  • Namita R, Kumar S, Jaganmohan S & Murugan V, DNA vaccines encoding viral envelope proteins confer protective immunity against WSSV in black tiger shrimp, Vaccine, 25 (15) (2007) 2778–2786.
  • Freshney R I, Culture of animal cells, a manual of basic technique, 5 th edn, (Wiley-Blackwell Publishers, New York), 2007, pp. 365–368.
  • Khuri A I & Cornell J A, Response Surfaces: Design and Analyses, (Marcel Dekker Inc., New York), 1987, pp. 105.
  • Montgomery D C, Analysis and Design of Experiments, 4 th edn, (Wiley, New York), 1991.
  • Kokare C R, Kadam S S, Mahadik K R & Chopade B A, Fermentation process optimization for bioemulsifier production of marine Streptomyces species S1, 7 th Asia Pacific Marine Biotechnol Conf., National Institute of Oceanography Kochi, India, 2006.
  • Kokare C R, Kadam S S, Mahadik K R & Chopade B A, Studies on bioemulsifier production from marine Streptomyces sp. S1, Indian J Biotechnol, 6 (2007) 78-84.
  • Kiran G S, Hema T A, Gandhimathi R, Selvin J, Anto Thomas T, et al., Optimization and production of a biosurfactant from the sponge-associated marine fungus Aspergillus ustus MSF3, Colloids Surf B: Biointerfaces, 73 (2) (2009) 250–256.
  • Kiran S, Thomos T A & Selvin J, Production of new glycolipid biosurfactant from marine Nocardiopsis lucentensis MSA04 in solid state cultivation, Colloids Surf B: Biointerfaces, 78 (1) (2010) 8-16.
  • Satpute S K, Bhawsar B D, Dhakephalkar P K & Chopade B A, Assessment of different screening methods for selecting biosurfactant producing marine bacteria, Indian J Geo-Mar Sci, 37 (3) (2008) 243–250.
  • Persson A & Molin G, Capacity for biosurfactant production of environmental Pseudomonas and Vibrionaceae growing on carbohydrates, Appl Microbiol Biotechnol, 26 (1987) 439–442.
  • Donio M B S, Ronica S F A, ThangaViji V, Velmurugan S, Adlin Jenifer J, et al., Isolation and characterization of halophilic Bacillus sp. BS3 able to produce pharmacologically important biosurfactants, Asia Pac J Trop Med, (2013) 876–883.
  • Jain R M, Mody K, Mishra A & Jha B, Isolation and structural characterization of biosurfactant produced by an alkaliphilic bacterium Cronobacter sakazakii isolated from oil contaminated wastewater, Carbohyd Poly, 87 (3) (2012) 2320–2326.
  • Li H, Liu H Y, Luo N, Zhang X Y, Luan T G, et al., Biodegradation of benzene and its derivatives by a psychrotolerant and moderately haloalkaliphilic Planococcus sp. strain ZD22, Res Microbiol, 157 (7) (2006) 629-636.
  • García M T, Mellado E, Ostos J C & Ventosa A, Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds, Int J Syst Evol Microbiol, 54 (5) (2004) 1723–1728.
  • Moreno M D L, Sanchez-Porro C, Piubeli F, Frias L, García M T, et al., Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans, PLoS ONE, 6 (2011) p. 21049.
  • Bertrand J C, Al-Mallah M, Acquaviva M & Mille G, Biodegradation of hydrocarbons by an extremely halophilic archaebacterium, Lett Appl Microbiol, 11 (5) (1990) 260-263.
  • Fairley D J, Boyd D R, Sharma N D, Allen C C R, Morgan P, et al., Aerobic metabolism of 4-hydroxybenzoic acid in archaea via an unusual pathway involving an intramolecular migration (NIH Shift), Appl Environ Microbiol, 68 (12) (2002) 6246–6255.
  • Sutherland I W, Biofilm exopolysaccharides: A strong and sticky framework, Microbiology, 147 (1) (2001) 3–9.
  • Bramhachari P V, Kishor P B, Ramadevi R, Kumar R, Rao B R, et al., Isolation and characterization of mucous exopolysaccharide (EPS) produced by Vibrio furnissii strain VBOS3, J Microbiol Biotechnol, 17 (1) (2007) 44–51.
  • Thavasi R, Subramanyam Nambaru V R M, Jayalakshmi S, Balasubramanian T & Banat I M, Biosurfactant Production by Azotobacter chroococcum isolated from the Marine Environment, Mar Biotechnol, 11 (5) (2009) 551–556.
  • Thavasi R, Jayalakshmi S, Balasubramanian T & Banat I, Biodegradation of crude oil by nitrogen fixing marine bacteria Azotobacter chroococcum, J Microbiol, 1 (5) (2006) 401–408.
  • Rosenberg M & Rosenberg E, Bacterial adherence at the hydrocarbon-water interface, Oil Petrochem Pollut, 2 (3) (1985) 155–162.
  • Joshi S, Bharucha C & Desai A J, Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B, Bioresour Technol, 99 (11) (2008) 4303-4603.
  • Anton J, Meseguer I & Rodriguez-Valera F, Production of an extracellular polysaccharide by Haloferax mediterranei, Appl Environ Microbiol, 54 (10) (1998) 2381–2386.
  • Austin B, Novel pharmaceutical compounds from marine bacteria, J Appl Bacteriol, 67 (5) (1989) 461–470.
  • Klykken P C, Galbraith T W & Kolesar G B, Jean P A, Woolhiser M R, et al., Toxicology and humoral immunity assessment of octamethylcyclotetrasiloxane (D4) following a 28-day whole body vapor inhalation exposure in Fischer 344 rats, Drug Chem Toxicol, 22 (4) (1999) 655-677.
  • Rathinamala J, Anjana J C, Sruthy P B & Jayashree S, Phytochemical screening and GC-MS analysis of bioactive compounds from Mimusops elengi, Annals Pharma Res, 1 (2) (2013) 32-34.
  • Felix K, Janz S, Pitha J, Williams J A, Mushinski E B, et al., Cytotoxicity and Membrane Damage in vitro by inclusion Complexes Between γ-Cyclodextrin and Siloxanes, Current Topics Microbiol Immuno, 210 (1996) 93-99.
  • Moustafa M F M, Alamri S A, Taha T H & Alrumman S A, In vitro antifungal activity of Argemone ochroleuca sweet latex against some pathogenic fungi, African J Biotechnol, 12 (10) (2013) 1132-1137.
  • Ganesh Kumar A, Vijayakumar L, Joshi G, Magesh Peter D, Dharani G, et al., Biodegradation of complex hydrocarbons in spent engine oil by novel bacterial consortium isolated from deep sea sediment, Biores Techno, 170 (2014) 556–564.
  • Rodrigues L R & Teixeira J A, Biomedical and Therapeutic Applications of Biosurfactants, Adv Exp Med Bio, 672 (2010) 75-87.
  • Kiran G S, Nishanth L A, Priyadharshini S, Anitha K & Selvin J, Effect of Fe nanoparticle on growth and glycolipid biosurfactant production under solid state culture by marine Nocardiopsis sp. MSA13A, BMC Biotechnology, 14 (2014) p. 48.
  • Donio M B S, Ronica S F A, ThangaViji V, Velmurugan S, Adlin Jenifer J, et al., Halomonas sp. BS4, A biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance, Springer Plus, 2 (1) (2013) p. 149.
  • Vollenbroich D, Ozel M, Vater J, Kamp R M & Pauli G, Mechanism of inactivation of enveloped viruses by the biosurfactant surfactin from Bacillus subtilis, Biologicals, 25 (3) (1997) 289–297.
  • Kim S Y, Kim J Y, Kim S H, Bae H J, Yi H, et al., Surfactin from Bacillus subtilis displays anti-proliferative effect via apoptosis induction, cell cycle arrest and survival signaling suppression, FEBS Lett, 581 (5) (2007) 865-71.
  • Raghavarao K S M S, Ranganathan T V & Karanth N G, Some engineering aspects of solid-state fermentation, Biochem Eng J, 13 (2-3) (2003) 127-135.
  • Patel R M & Desai A J, Biosurfactant production by P. aeruginosa GS3 from molasses, Lett Appl Microbiol, 25 (1997) 91-94.
  • Abouseoud M, Maachi R, Amrane A, Boudergua S & Nabi A, Evaluation of different carbon and nitrogen sources in production of biosurfactant by Pseudomonas fluorescens, Desalination, 223 (1-3) (2008) 143-151.
  • Elazzazy A M, Abdelmoneim T S & Almaghrabi O A, Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia, Saudi J Bio Sci, 22 (4) (2014) 466–475.

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  • Characterization and biomedical application of Tetradecamethylcycloheptasiloxane, a silicone-type biosurfactant produced by Streptomyces castaneoglobisporus AJ9 isolated from solar salt works

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Authors

M B S Donio
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Tamilnadu – 629 502, India., India
R Remya
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Tamilnadu – 629 502, India., India
M Michaelbabu
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Tamilnadu – 629 502, India., India
G Uma
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Tamilnadu – 629 502, India., India
T Citarasu
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Tamilnadu – 629 502, India., India

Abstract


Tetradecamethylcycloheptasiloxane (C14H42O7 Si7 ), a biosurfactant, was extracted by acid precipitation method from the haloalkaliphilic actinomycetes, Streptomyces castaneoglobisporus AJ9 (Gene Bank KC603900.1) and characterized by GC-MS analysis. The purified biosurfactant could effectively degrade dyes such as orange MR, direct violet, cotton red, reactive yellow and nitro green. Antimicrobial screening results showed that biosurfact ants could effectively control bacterial pathogens such as Staphylococcus aureus and Vibrio parahaemolyticus and fungal pathogens such as Fusarium sp. and Penicillium sp. Even a small diminutive quantity of biosurfactant (100 μg) could totally block the multiplication of White Spot Syndrome Virus (WSSV) in shrimp in vivo. The suppression of cancer cells in vitro by the biosurfactant was 74 % as confirmed by Response Surface Methodology (RSM) and residual plot analysis. Wheat bran, groundnut oilcake and oilseed cake seem to enhance biosurfactant production.

Keywords


Antimicrobials, Biosurfactants, Streptomyces castaneoglobisporus, Tetradecamethylcycloheptasiloxne.

References