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Molecular Characterization and Identification of Unknown Bacteria from Waste Water


Affiliations
1 Department of Biotechnology, Hindustan College of Arts & Science, Padur, Kelambakkam -603103, India
 

Water plays a very important role in supporting all forms of life. However if it contaminated, it has a great potential for transmitting a variety of diseases and illnesses. Waste water can serve as a source of infectious bacteria, viruses and other pathogens. The transmission of these diseases through waste water is a constant public threat. The purpose of this study was to identify the unknown bacteria, which cause dreadful disease to humans. The sample was collected from household waste water and the organism was screened using selective media and the taxonomy status was identified based on biochemical test. The molecular characterization is done by isolating DNA from the selected organism. Universal primer was designed based on 16srRNA. The conserved region was amplified and the amplified product was sequenced based on 16s rRNA.

Keywords

Molecular Biology, Bacterial Characterization, Waste Water, sRNA
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Abstract Views: 1073

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  • Molecular Characterization and Identification of Unknown Bacteria from Waste Water

Abstract Views: 1073  |  PDF Views: 230

Authors

V. Brindha
Department of Biotechnology, Hindustan College of Arts & Science, Padur, Kelambakkam -603103, India
Ani Ann Mathew
Department of Biotechnology, Hindustan College of Arts & Science, Padur, Kelambakkam -603103, India

Abstract


Water plays a very important role in supporting all forms of life. However if it contaminated, it has a great potential for transmitting a variety of diseases and illnesses. Waste water can serve as a source of infectious bacteria, viruses and other pathogens. The transmission of these diseases through waste water is a constant public threat. The purpose of this study was to identify the unknown bacteria, which cause dreadful disease to humans. The sample was collected from household waste water and the organism was screened using selective media and the taxonomy status was identified based on biochemical test. The molecular characterization is done by isolating DNA from the selected organism. Universal primer was designed based on 16srRNA. The conserved region was amplified and the amplified product was sequenced based on 16s rRNA.

Keywords


Molecular Biology, Bacterial Characterization, Waste Water, sRNA

References