Indian Journal of Innovations and Developments

Open Access Open Access  Restricted Access Subscription Access

Saliva - a Marker for the Diabetic: a Comparative Study of Healthy and the Diabetic Individuals


Affiliations
1 Department of Biotechnology, Hindustan College of arts & Science, Kelambakkam-603103, Tamil Nadu, India
 

Saliva contains a wide range of proteins, antibodies, nucleic acids that makes it an important diagnostic fluid. The most effective non-invasive source of DNA, RNA and protein is saliva, which comes from buccal epithelial cells. The saliva from the healthy and the diabetic individuals was collected with volunteers concern. We quantified the protein content spectrophotometrically and it was found that the protein was decreased in the diabetic individual. The protein was resolved in SDS-PAGE and the protein profiles of whole saliva of healthy and diabetic were compared. Considerable variations between individuals were observed. The spectrophotometric quantification of sialic acid showed a significant increase in its levels in diabetic individuals as compared with non-diabetics.

Keywords

Saliva, Diabetics, Comparative Study, Spectrophotometry, Metabolic Disorder
User
Notifications

  • Abdella N, Akanji AO, Mojiminiyi OA, Assoussi AA and Moussa M (2000) Relation of serum total sialic acid concentrations with diabetic complications and cardiovascular risk factors in Kuwaiti Type 2 diabetic patients. Diabetes Research and Clinical Practice 50, 65- 72.

  • Chiappen S, Antonelli G, Gatti R and Palo EF (2007) Saliva specimen: a new laboratory tool diagnostic and basic investigation. Clin chim Acta, 38, 30-40. 3. Daniel PK, David K, Serena Choo and Chia KS (2006) Saliva as a viable alternative source of human genomic DNA in genetic epidemiology. Clinical Chimica Acta, 367, 81-85.

  • Hallikeri K, Sanjay PR and Shivashankara AR (2008) Evaluation of salivary sialic-acid, total protein, and total sugar in oral cancer: A preliminary report. Indian J Dent Res, 19, 288-91.

  • Hoe AZ, Pendek R, Lam SK and Rahim ZHA (1997) Preliminary study on from diabetic and non-diabetic individuals by polyacrylamide gel electrophoresis. Annals Dent 4, 7-8.

  • Huang CM (2004) Comparitive proteomic analysis of human whole saliva. Archives of Oral Biology 49, 951- 962.

  • Jiang Jiang, Noh Jin Park, Shen Hu and David T Wong (2009) A universal pre-analytic solution for concurrent stabilization of salivary proteins, RNA and DNA at ambient temperature. Oral biology, 54, 268-273.

  • Kanto EA, Hirata MH, Hirata RD, Wunes FD, Hattenhoff RF and Oliveira MR (2005) DNA extraction from human saliva deposited on skin and its use in forensic identification procedures. Oral biology, 56, 119-128.

  • Ghitescu DL, Gugliucci A and Dumas F (2001) Actin and Annexins I and II Are among the Main Endothelial Plasmalemma-Associated Proteins Forming early Glucose Adducts in Experimental Diabetes. Journal of diabetics, 50, 1666-1674.

  • Sachwark NV, Malysz V, Fremdt H, Koch C, Simeoni E and Schwark T (2006) Fast and simple DNA extraction from saliva and sperm cells obtained from skin or isolated from swabs. Legal Medicine 8, 177-181.


Abstract Views: 349

PDF Views: 78




  • Saliva - a Marker for the Diabetic: a Comparative Study of Healthy and the Diabetic Individuals

Abstract Views: 349  |  PDF Views: 78

Authors

J. Kumar
Department of Biotechnology, Hindustan College of arts & Science, Kelambakkam-603103, Tamil Nadu, India

Abstract


Saliva contains a wide range of proteins, antibodies, nucleic acids that makes it an important diagnostic fluid. The most effective non-invasive source of DNA, RNA and protein is saliva, which comes from buccal epithelial cells. The saliva from the healthy and the diabetic individuals was collected with volunteers concern. We quantified the protein content spectrophotometrically and it was found that the protein was decreased in the diabetic individual. The protein was resolved in SDS-PAGE and the protein profiles of whole saliva of healthy and diabetic were compared. Considerable variations between individuals were observed. The spectrophotometric quantification of sialic acid showed a significant increase in its levels in diabetic individuals as compared with non-diabetics.

Keywords


Saliva, Diabetics, Comparative Study, Spectrophotometry, Metabolic Disorder

References