Indian Journal of Innovations and Developments

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Isolation and Analysis of Proteins in Shrimp Waste


Affiliations
1 Department of Biotechnology, Hindustan College of arts and science Padur, Chennai-600073, Tamil Nadu, India
2 Department of Biotechnology, Hindustan College of arts and science Padur, Chennai-600073, Tamil Nadu, India
 

The objective of this study is to measure and quantify the proteins present in shrimp waste which is estimated by lowrys protein assay after the extraction of the proteins from the shrimp waste using MLB lysis buffer. The correlation of the amino acids present in it are found by using thin layer chromatography technique, then the peptides present in the proteins are separated out by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and native polyacrylamide gel electrophoresis.

Keywords

Protein Isolation, Shrimp Wast, Thin-layer Chromatography, Protein Analysis
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  • Isolation and Analysis of Proteins in Shrimp Waste

Abstract Views: 321  |  PDF Views: 68

Authors

R. Eswaralakshmi
Department of Biotechnology, Hindustan College of arts and science Padur, Chennai-600073, Tamil Nadu, India
K. Jeyandra Saravanan
Department of Biotechnology, Hindustan College of arts and science Padur, Chennai-600073, Tamil Nadu, India

Abstract


The objective of this study is to measure and quantify the proteins present in shrimp waste which is estimated by lowrys protein assay after the extraction of the proteins from the shrimp waste using MLB lysis buffer. The correlation of the amino acids present in it are found by using thin layer chromatography technique, then the peptides present in the proteins are separated out by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and native polyacrylamide gel electrophoresis.

Keywords


Protein Isolation, Shrimp Wast, Thin-layer Chromatography, Protein Analysis

References