Indian Journal of Innovations and Developments

Open Access Open Access  Restricted Access Subscription Access

In Vitro Multiplication and Phyto-chemical Analysis of a Valuable Medicinal Plant Withania Somnifera L. Dunal


Affiliations
1 Department of Botany and Biotechnology, Laboratory of Plant Tissue Culture, Government E. Raghvendra Rao P. G. College of Science, Bilaspur - 495006, Chhattisgarh, India
2 Government Nagarjuna, P. G. College of science, Raipur - 492010, Chhattisgarh, India
 

Withania somnifera, a popular Indian medicinal plant has long been used commonly in ayurvedic system of medicine. All the parts of the plants have shown remarkable importance in the field of pharmacology. The present paper gives an account of information on in-vitro micro propagation of medicinally important plant for the generation of multiple shoots and ischolar_mains of Withania somnifera and its phyto-chemical activities. Shoots were induced from the axillary buds of Withania somnifera on Murashige and Skoogs's (MS) basal media supplemented with different concentrations of BAP (0.10mg/l, 0.25mg/l, 0.50mg/l, 0.75mg/l, 1.00mg/l). Axillary bud explants showed initiation of shoots with the concentrations of 1mg/l and it is found to be most effective for multiple shoot generation within minimum time period. The leaf, stem and ischolar_main of Withania somnifera were analyzed for phyto-chemical screening. The micro propagated plants were transplanted to pots for hardening.

Keywords

In-vitro Multiplication, MS Medium, Withania somnifera, Shoot Bud Culture, Benzyl Amino Purine, Axiliary
User
Notifications

  • Bapat VA, Yadav SR and Dixit GB (2008) Rescue of endangered plants through biotechnological applications, Natt, Acad. Sci. Lett., 31, 201-210.

  • Bhattacharya R and Bhattacharya S (2001) High frequency of in vitro propagation of Phyllanthus amarus by shoot tip culture. Indian J. Exp. Biol., 39, 1184-1187.

  • Catapan E, Otuki MF and Viana AM (2000) In vitro culture of Phyllanthus caraliniensis. Plant Cell Tiss. Organ Cult. 62, 195-202.

  • Dube P, Gangopadhyay M, Deewanjee S and Ali MN (2011) Establishment of a rapid multiplication protocol of Coleus forskohlii Briq and in vitro conservation by reduced growth. Indian J. Biotechnol., 10, 228-231.

  • Fay MF (1992) Conservation of rare and endangered plants using in vitro methods. In Vitro Cell Dev. Biol. Plant. 28, 1-4.

  • Kirtikar KR and Basu BD (2001) In: Basu LM ed. Indian Medicinal Plants. Oriental Enterprises, Allahabad, India, Vol. 8, 2445-49

  • Laxshami GN and Seeni S (2001) Rapid in vitro multiplication and restoration of Celastrus paniculatus Willd. Subsps. Paniculatus (Celastraceae), a medicanal woody climber. Indian J. Exp. Biol. 39, 697-704.

  • Mohamed IE, Nur El and Abdelrahman ME (2010) The antibacterial, antiviral activities and phytochemical screening of some Sudanese medicinal plants, Journal BioSci, 4, 8-16.

  • Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology Plant. 15, 473-97.

  • Nagesh KS and Shanthamma C (2011) Micropropagation and antioxidant activity of Mollugo nudicaulis lam. J. Med. Plants Res. 5(6), 895-902.

  • Sahoo Y, Pattnaik SK and Chand PK (1997) In vitro clonal propagation of an aromatic medicinal herb Ocimum basilicum L. (Sweet basil) by axillary shoots proliferation. In vitro cell, Devel. Biol. Plant Largo, 33, 293-296.

  • Siddique I and Anis M (2007) Rapid micropropagation of Ocimum basilicum using shoot tip explants pre-cultured in thidiazuron supplemented liquid medium. Biol. Plant. 51, 787-790.

  • Tripathy AK, Shukla YN and Kumar S (1996) Ashwagandha [Withania somnifera Dunal (Solanaceae)]: A status report. J. Med. & Aromat. Plant Sci., 18, 46-62.


Abstract Views: 372

PDF Views: 68




  • In Vitro Multiplication and Phyto-chemical Analysis of a Valuable Medicinal Plant Withania Somnifera L. Dunal

Abstract Views: 372  |  PDF Views: 68

Authors

Prerna Soni
Department of Botany and Biotechnology, Laboratory of Plant Tissue Culture, Government E. Raghvendra Rao P. G. College of Science, Bilaspur - 495006, Chhattisgarh, India
A. N. Bahadur
Government Nagarjuna, P. G. College of science, Raipur - 492010, Chhattisgarh, India
U. Tiwari
Government Nagarjuna, P. G. College of science, Raipur - 492010, Chhattisgarh, India
V. K. Kanungo
Government Nagarjuna, P. G. College of science, Raipur - 492010, Chhattisgarh, India

Abstract


Withania somnifera, a popular Indian medicinal plant has long been used commonly in ayurvedic system of medicine. All the parts of the plants have shown remarkable importance in the field of pharmacology. The present paper gives an account of information on in-vitro micro propagation of medicinally important plant for the generation of multiple shoots and ischolar_mains of Withania somnifera and its phyto-chemical activities. Shoots were induced from the axillary buds of Withania somnifera on Murashige and Skoogs's (MS) basal media supplemented with different concentrations of BAP (0.10mg/l, 0.25mg/l, 0.50mg/l, 0.75mg/l, 1.00mg/l). Axillary bud explants showed initiation of shoots with the concentrations of 1mg/l and it is found to be most effective for multiple shoot generation within minimum time period. The leaf, stem and ischolar_main of Withania somnifera were analyzed for phyto-chemical screening. The micro propagated plants were transplanted to pots for hardening.

Keywords


In-vitro Multiplication, MS Medium, Withania somnifera, Shoot Bud Culture, Benzyl Amino Purine, Axiliary

References