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Exogenous Salicylic Acid Alleviates Arsenic Toxicity in Arabidopsis thaliana


Affiliations
1 Department of Botany, University of Lagos, Akoka, Lagos, Nigeria
 

The present study investigated the possible protective role of salicylic acid (SA) against arsenic toxicity in Arabidopsis thaliana. Seedlings were raised from seeds in plastic containers filled with commercial propagation medium and were divided into two groups. Half of the seedlings were treated with 250 μM salicylic acid for 2 weeks, after which both groups were exposed to 100 μM arsenic for 2 weeks. Metabolic parameters representative of oxidative damage and antioxidant activity were evaluated after the treatments. The result showed that arsenic caused a decrease in plant biomass, chlorophyll content and a significant increase in lipid peroxidation, and activities of catalase, ascorbate peroxidase and superoxide dismutase in seedlings that were not pretreated with SA. The toxic effects of arsenic were however alleviated by the exogenously applied SA thereby underscoring the beneficial role of this signal molecule in mediating defense response in plants under stress.

Keywords

Antioxidant Enzyme, Metals, Oxidative Stress, Signal Molecule, Pollution, Environment
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  • Exogenous Salicylic Acid Alleviates Arsenic Toxicity in Arabidopsis thaliana

Abstract Views: 428  |  PDF Views: 60

Authors

Victor Johwo Odjegba
Department of Botany, University of Lagos, Akoka, Lagos, Nigeria

Abstract


The present study investigated the possible protective role of salicylic acid (SA) against arsenic toxicity in Arabidopsis thaliana. Seedlings were raised from seeds in plastic containers filled with commercial propagation medium and were divided into two groups. Half of the seedlings were treated with 250 μM salicylic acid for 2 weeks, after which both groups were exposed to 100 μM arsenic for 2 weeks. Metabolic parameters representative of oxidative damage and antioxidant activity were evaluated after the treatments. The result showed that arsenic caused a decrease in plant biomass, chlorophyll content and a significant increase in lipid peroxidation, and activities of catalase, ascorbate peroxidase and superoxide dismutase in seedlings that were not pretreated with SA. The toxic effects of arsenic were however alleviated by the exogenously applied SA thereby underscoring the beneficial role of this signal molecule in mediating defense response in plants under stress.

Keywords


Antioxidant Enzyme, Metals, Oxidative Stress, Signal Molecule, Pollution, Environment

References