Indian Journal of Innovations and Developments

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Staining with lactophenol and methylene blue is a simple and single step, at the same time it is a convincing method for the microscopic identification of fungal mycelial structures. This study describes the preparation and extraction of natural dye from Roselle (Hibiscus sabdariffa) calyx for single staining techniques of fungal mycelial structure, as well as its staining effect on fungal sporangia. Various treatments using the plant (calyx) aqueous extract pH 1.9 (Temp. 30.1°C), and ethanolic extract pH 2.9 (Temp. 30.1°C), were carried out on different fungal spp, viz: Aspergillus nidulans, Rhizoctonia sp, Rhizopus sp, Penicillium ctrinum, Cladosporium spp and Mucor spp. Different levels of differentiation of the fungal mycelial structures were obtained, using simple staining techniques and viewed under low and high power objectives of Carl zeiss microscope. All the extracts had the affinity to stain fungal mycelia as well as the fungal sporangia, the fungal mycelia and sporangia are stained red and stood out well against fairly red background. The wavelength with highest absorbance was obtained at 460nm for the extracts using scan ultraviolent spectrophotometer model st-uv-755v.

Keywords

Hibiscus sabdariffa (linn), Potassium Alum, Staining, Fungal Sporangia
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