Toxicity of copper (Cu) and lead (Pb) was evaluated using Allium cepa L. Healthy onion bulbs of relatively equal size were grown for 3 weeks in half-strength Hoagland's solution spiked with copper and lead individually to achieve 0.1, 0.3, and 0.5 mM of the metals. Nutrient solution devoid of these metals served as control. Parameters investigated include ischolar_main length, number of ischolar_mains, lipid peroxidation, and cytogenetic study. The results of this study indicate that these metals consistently inhibited ischolar_main growth, induced chromosomal aberration, and increased lipid peroxidation. The study also revealed that the effects were concentration dependent. It was found in this study that A. cepa was very sensitive to copper and lead, suggesting usage as indicator to monitor pollution by these metals of the environment.
Keywords
Heavy Metals, Oxidative Stress, Lipid Peroxidation, Chromosome Aberration, Allium Cepa
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