Indian Journal of Medicine and Healthcare

Open Access Open Access  Restricted Access Subscription Access

Hepatoprotective Effect of Methanolic Leaf Extract of Boswellia dalzielii hutch on Carbon Tetrachloride Induced Hepatotoxicity in Wistar Rats


Affiliations
1 Department of Biochemistry, Faculty of science, University of Port Harcourt, P.M.B 5323, Port Harcourt, River state, Nigeria
 

The aqueous extract of Boswellia dalzielii Hutch was reported to strengthen the liver. Hence, the effect of this plant on carbon tetrachloride (CCL4) induced hepatotoxicity was investigated. 96 male rats were divided into 7 groups of 12 rats each. 100mg/kg, 200mg/kg and 300mg/kg methanolic extract of the leaves of Boswellia dalzielii Hutch were administered orally for 4 weeks after 0.63ml of CCl4/kg body weight was used to induce hepatotoxicity. Reducdyn and vitamin E were used as standard control drugs. Liver functions were assessed by the assay of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), albumin (ALB), total bilirubin (TB) and thiobarbituric acid reactive substances (TBARS). The biochemical observations were confirmed by histological examinations of the liver sections and are comparable with the standard hepatoprotective drug, reducdyn and vitamin E, which served as positive control. The effect of the extract increased with increase in the extract concentration. When the different concentrations of the extract were compared with the drugs, they were found to mimic the hepatoprotective effect of the drugs. The overall experimental results indicate that the biologically active antioxidant phytoconstituents of the methanolic extract of Boswellia dalzielii Hutch could alleviate the toxic action of CCl4 in the liver of rat.

Keywords

Boswellia dalzielii Hutch, carbon Tetrachloride, Hepatotoxicity, Histopathology, Liver Function Enzymes, Reducdyn,vitamin E, Anti-oxidants.
User
Notifications

  • Aliyu R, Gatsing D and Jaryum KH (2007) The effect of Boswellia dalzielii (Buseraceae) aqueous bark extract on rat liver function. Asian J. Biochem. 2(5), 359-363.

  • Burkill HM (1985) Useful plants of West Tropical Africa. Vol.1, Royal Botanical Gardens Kew. pp:300.

  • Burtis CA and Ashwood ER (2001) Tiez fundamentals of clinical chemistry. (5th ed) W. B. Saunders Company, Philadelphia. pp: 352-768.

  • Chattopadhyay RR (2003) Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract Part II. J. Ethnophar. Macol. 89,217-219.

  • Crook MA (2006) Clinical chemistry and metabolic medicine. 7th Edn. Arnold Ltd. pp:253.

  • Dimas k, Ishaka AO, Domingo AO, Nureni OO and Adeleke AK (2006) Constituents of the essential oils of Boswellia dalzielii Hutch from Nigeria. J. Essential oil Research.

  • Ethuk EU, Agale BM, Onyeyili PA and Ottah CU (2006) Toxicological studies of aqueous stem bark extract of Boswellia dalzielii in albino rats. Indian J. Pharmacology, 38:359-360.

  • Gurusamy K, Kokilavani R and Arumuasamy (2010) Hepatoprotective activity of polyherbal formulation against CCl4 induced hepatoxicity in rats. African J. Biotechnology, 9(49),8429-8434.

  • Hassan HS, Musa AM, Usman MA and Abdulaziz M (2009) Preliminary phytochemical and antispasmodic studies of the stem bark of Boswellia dalzielii. Nigerian J. Pharmaceutical Sci. 8(1),1-6.

  • Jeong TC, Gu HK, Park J, Yun H, Kim HC, Sulta C and Roh JK (1999) Pretreatment of male BALB/c mice &- ionona Potentiates thioacetamide induced hepatotoxicity. Toxicology letter.105,39-46.

  • Krishna KL, Mruthunjaya K and Patel JA (2011) Antioxidant and hepatoprotective potential of stem methanolic extract of Justica gendarussa. Intl. Pharmacology. 6,78-80.

  • Kumar BS, Sathyanarayana J, Krishnareddy M and Prasad MSK (2011) Evaluation of hepatoprotective activity of aqueous extract from leaves of Solanum americanum. Intl. J. Plant, Animal & Environmental Sciences, 1(2),150-154.

  • Mascolo N, Sharma R, Jain SC and Capasso F (1998) Ethnopharmacology of Calotropis procera flowers. J. Ethnopharmacology. 22, 211-244.

  • Mitra SK, Venkataranganna MV, Sundaram R and Gopumadhavan S (1998) Protective effect of HD-03, a herbal formulation, against various Hepatotoxic agents in rats. J.Ethnopharmacol, 63:181-186.

  • Mossa JS, Tariq M, Mohsin A, Aqueel AM, Al-Yahya MA, Al-said MS and Rafatullah S (1991) Pharmacological studies on aerial parts of Calotropis procera. Amer. J. Clinical Med.19,223-231.

  • Muriel P and Garcipiana T (1992) Silimarin protects against paracetamol induced lipid peroxidation and liver damage. J. Applied Toxicology.12,439-442.

  • Nakagawa Y, Moldeus P and Moore GA (1992) Cytotoxin of orthophenol in isolated rat hepatocyte. Biochem. & Pharmacology J. 43,159-165.

  • Nwinyi FC, Binda L, Ajoku GA, Aniagu SO, Enwerem NM, Orisadipe A, Kubmarawe D and Gamaniel KS (2004) Evaluation of the aqueous extract of Boswellia dalzielii Stem bark for antimicrobial activities and gastro intestinal effects. African Journal of Biotechnology, 3(5), 284-288.

  • Obi FO, Useni IA and Osayande JO (1998) Prevention of CCl4 induced hepatoxicity (H. rosasinensis ). Food Chemistry. 31, 9-18.

  • Ohkawa H, Ohishi N and Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95,351-358.

  • Patrick-Iwuanyanwu KC, Onyeinke EN and Wegwu MO (2010) Hepatoprotective effect of methanolic extract and fractions of Afeican mistletoe (Tapinanthus bangwensis) from Nigeria. EXCLI J. 9,187-194.

  • Patric-Iwuanyanwu KC and Wegu MO (2008) Prevention of carbon tetrachloride induced liver damage in rats by Acanthus montanus. Asian J. Biochem. 3,213-220.

  • Patric-Iwuanyanwu KC and Wegu MO, Ayalogu EO (2007) Prevention of carbon tetrachloride induced liver damage by ginger, galic and vitamin E. Parkinstan J. Biological Sci.10,617-621.

  • Reitman S and Frankel S (1975) Invitro determination of transaminase activity in serum. Amer. J. Clinical Pathology. 28,56-58.

  • Rekha R, Hemalatha S, Akasakalai K, Mudhukrisna CH, Vittal SB and Sundaram RM (2009) Hepatoprotective activity of Mimosa pudica leaves against CCl4 induced toxicity. J. Natural Products. 2,116-122.

  • Schuppan DJ, Brikhaus B, Hahn EG (1999) Herbal products for liver disease: A therapeutic challenge for the new millennium. Heamatology J. 30,1099- 1104.

  • Sofowora EA (1984) Medical plants and traditional medicine in Africa. John Willey, Chicaster, pp: 256.

  • Strinath A, Jyothi V and Asha JV (2010) Hepatoprotective activity. Intl J. Pharm. & Technol. 2(3),354-366.

  • Surest–Kumar SV and Mistra SH (2008) Hepatoprotective effect of Pergularia daemia ethanol extract and its fraction. Indian J. Experimental Biology.46, 447-452.

  • Trease GE and Evans WC (1978) Pharmacognosy. 11th Edn. Bailliere Tindall, London, pp:176-180.

  • Uliena O, Greksak M, Vancova O, Zlatos L, Galbary S, Bozek P and Nakano M (2003) Hepatoprotective effect of Rooibos tea (Aspalathus linearis) on CCl4 induced liver damage in rats. Physiology Res. 52,461- 466.

  • Venukumar MR and Latha MS (2002) Hepatoprotective effect of the methanolic extract of of orchids in CCl4 treated male rat. J. Pharmacology. 34,2.

  • Yadav MS, Kumar A, Sigh A, Sharma US and Sutar N (2011) Phytochemical investigation and hepatoprotective activity of Cissampelos pariera against CCl4 induced hepatotoxicity. Asian Journal of Pharmaceutical & Health Sci. 1(3),106-110.

  • Yan-Jun I, Jie-Ping Y, Zhao-Hong S and Wang L (2004) Gingkgo biloba extracts reverses CCl4 induced liver fibrosis in rats. World J. Gastroentrology, 10,1037-1042.


Abstract Views: 521

PDF Views: 493




  • Hepatoprotective Effect of Methanolic Leaf Extract of Boswellia dalzielii hutch on Carbon Tetrachloride Induced Hepatotoxicity in Wistar Rats

Abstract Views: 521  |  PDF Views: 493

Authors

D. A. Onoriose
Department of Biochemistry, Faculty of science, University of Port Harcourt, P.M.B 5323, Port Harcourt, River state, Nigeria
A. A. Uwakwe
Department of Biochemistry, Faculty of science, University of Port Harcourt, P.M.B 5323, Port Harcourt, River state, Nigeria
C. C. Monago
Department of Biochemistry, Faculty of science, University of Port Harcourt, P.M.B 5323, Port Harcourt, River state, Nigeria
O. B. Odeghe
Department of Biochemistry, Faculty of science, University of Port Harcourt, P.M.B 5323, Port Harcourt, River state, Nigeria

Abstract


The aqueous extract of Boswellia dalzielii Hutch was reported to strengthen the liver. Hence, the effect of this plant on carbon tetrachloride (CCL4) induced hepatotoxicity was investigated. 96 male rats were divided into 7 groups of 12 rats each. 100mg/kg, 200mg/kg and 300mg/kg methanolic extract of the leaves of Boswellia dalzielii Hutch were administered orally for 4 weeks after 0.63ml of CCl4/kg body weight was used to induce hepatotoxicity. Reducdyn and vitamin E were used as standard control drugs. Liver functions were assessed by the assay of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), albumin (ALB), total bilirubin (TB) and thiobarbituric acid reactive substances (TBARS). The biochemical observations were confirmed by histological examinations of the liver sections and are comparable with the standard hepatoprotective drug, reducdyn and vitamin E, which served as positive control. The effect of the extract increased with increase in the extract concentration. When the different concentrations of the extract were compared with the drugs, they were found to mimic the hepatoprotective effect of the drugs. The overall experimental results indicate that the biologically active antioxidant phytoconstituents of the methanolic extract of Boswellia dalzielii Hutch could alleviate the toxic action of CCl4 in the liver of rat.

Keywords


Boswellia dalzielii Hutch, carbon Tetrachloride, Hepatotoxicity, Histopathology, Liver Function Enzymes, Reducdyn,vitamin E, Anti-oxidants.

References