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Background/Objectives: Green nanotechnology is generating attention of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, the possible role of Sphaerostephanos asplenioides J. Sm extract in reducing silver nitrate into silver nanoparticle is highlighted.

Methods/Statistical analysis: A comparative study was made in preparing the silver nanoparticles using the boiling method and microwave irradiation method for the S. asplenioides. The synthesis of silver nanoparticles were prepared by adding silver nitrate solution [3mM] and [5mM] to the plant extract. The silver nanoparticles were characterized using the UV-Visible Spectroscopy.

Findings: The antimicrobial assay was carried out using the disc diffusion method which showed promising antibacterial effects against the 15 tested microorganisms. The phytochemical constituent determination shows the positive results expect for the test for terpenoids and test for the reducing sugar.

Improvements/Application: It can be concluded that both methods (Boiling Method and Microwave Irradiation Method) are effective in synthesizing of silver nanoparticles using the leaves extracts of plants.


Keywords

Sphaerostephanos asplenioides J. Sm., Antimicrobial Activity, Silver Nanoparticles, Silver Nitrate, Microwave Irradiation And Boiling Method.
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