Evaluation of Antimicrobial Effect of Conventional Calcium Hydroxide, Calcium Hydroxide Nanoparticle and Combined Calcium Hydroxide with Silver Nanoparticle as Intracanal Medication against Enterococcus Faecalis

Aim: This study was performed to evaluate the effect of three intracanal edicament Conventional calcium hydroxide (Ca(OH)₂), Ca(OH)₂ nanoparticle and Combined Ca(OH)₂ with silver nanoparticle on E.faecalis bacterial biofilm within infected ischolar_main canals. Forty five extracted human single ischolar_mained teeth were sterilized followed by mechanical preparation Microbiological culturing of E. faecalis was performed and incubated for 7 days. After incubation period, proper irrigation and premedication bacteriological sample was taken teeth were randomly divided according to the intracanal medicament used, samples were incubated in 37°C for 7 days. Second microbiological sample was taken after 7 days and culturing was done to allow bacterial count reduction. The non-parametric Kruskal-Wallis test was performed to evaluate the bacterial count reduction and percent of reduction at significance level was set to p ≤ 0.05.

Results: The antibacterial effect of combination of calcium hydroxide with silver nanoparticle was better than calcium hydroxide nanoparticle and followed by Conventional calcium hydroxide against E. faecalis bacteria.

Conclusion: combination Ca(OH)₂ with silver nanoparticle can be used instead of Conventional Ca(OH)₂ against E. faecalis bacteria in necrotic ischolar_main canals.