Gene Analysis Polymorphisms INSR and Level SHBG, as Risk Factors in Polycystic Ovary Syndrome (PCOS) in Madurese

PCOS (Polycystic ovary syndrome) one of the endocrine disorders most often in women of reproductive age, marked menstrual disorders (amenorrhea/oligomenorrhea), hirsutism, acne, alopecia, biochemical examination results in increased androgen. The high incidence with the same phenotype in family members of PCOS patients shows that genetic factors play a role. The purpose of this study was to analyze the relationship between gene polymorphism INSR and levels SHBG on PCOS (Polycystic ovary syndrome) in the Madurese tribe.

This study was analytic observational with study design case-control, a sample of 30 patients (PCOS cases) and 30 healthy women (not PCOS) in the Madura Tribe Previously, screening of SGOT, SGPT, BUN, Creatinin and GDA, PCR and RFLP was examined in the INSR gene and examination of SHBG levels by ELISA kit.gene research results INSR have 16.67% TT genotype, 63.33% TC and 20.00% CC, control group has 38.24% TT genotype, TC 44.12% and CC 17.65%, whereas in the case group T 48.33%, C allele 51.67% and control group 56.67% T allele and 43.33% C allele, based onstatistical tests chi-square in the case group and control of both genotypes and alleles there was no significant difference (P> 0.05). This study was first carried out in the Madura Tribe in the INSR gene and in the examination of SHBG levels, the results showed no INSR gene differences between PCOS and healthy women.