Antibacterial Activity of Zinc Oxide Nanoparticles on the Growth of Enterococcus Feacales, Candida and Total Root Canal Microbiota (in Vitro Study)

Severalendodontic irrigants,medication can eliminate endodontic pathogens,the possible development of resistant bacterial species, the anatomic complexity of the ischolar_main canal system and limitation of cleaning and shaping protocols making the achieving of complete elimination of the pathogens is difficult. Antimicrobial nanoparticles show promising effect against pathogens The concept of using nanoparticles in endodontics as a new treatment modality was developed recently and their antibacterial efficacy against endodontic pathogens was evaluated by several researchers in many in vitro studies. The aim of this study to evaluate the antimicrobial activity of zinc oxide nanoparticles against E.feacales, candida and total ischolar_main canal microbiota.30 patients diagnosed with necrotic ischolar_main canal and failed endodontic treatments was selected, microorganisms were isolated from ischolar_main canals andcultured, After identification and isolation of bacterial species, agar diffusion method was used to assess the antibacterial action of ZNO NPs, Zones of inhibition (no growth of bacteria) were examined around the wells containing ZNO NPs & diameters of the zones were measured in mm. The mean of inhibition zones was measured and statistically analyzed among groups using ANOVA. Znonps showed increase in diameter of the inhibition zone as the concentration increased, 2% concentration showed the larger inhibition zone, When comparing between the diameter of the inhibition zone of the ZNO NPs on the bacteria using F-test for all concentration the result was highly significant except for 0.25% concentration it was non-significant. It can be conclude that the zinc oxide nanoparticles in different concentrations have inhibition effects on E. feacales, candida, T. aerobic and T. anaerobic, the sensitivity of the bacteria to ZnO NPs increase with increasing of the concentration.