Comparison of Coliform, E. Coli, E. Coli O157, and E. Coli O157:H7 Bacteria in Bali Cattle in Mengwi, Bali

This study aims to determine the comparison of the number of Coliform, E. coli, E. coli O157, and E. coli O157:H7 bacteria in Bali cattle in Mengwi, Bali. Samples were taken as many as 58 faecal samples, which were further identified through several stages, namely growth on the eosin methylene blue agar media, Gram staining, indole test, methyl red, vogesproskauer, citrate, growth on sorconolMacConkey agar media, serological tests with E. coli O157 latex agglutination test and serological test with E. coli Anti-serum H7. From 58 Bali cattle feces samples taken, 100% were positive for Coliform, 55.1% were positive for E. coli, 6.8% were positive for E. coli O157, and 3.4% were detected as positive for E. coli O157:H7. Spearman’s rho analysis results showed that the high Coliform and E. coli bacteria did not significantly affect the discovery of E. coli O157 and E. coli O157:H7 bacteria. The conclusion that can be drawn is that from 58 faecal samples taken, 32 were positive samples of E. coli bacteria, whereas only 2 positive samples contained E. coli O157:H7 bacteria which were harmful to human health.