Immunohistochemical Expression of GLUT-1 and Carbonic Anhydrase IX in Major and Minor Pleomorphic Adenoma PF the Salivary Glands

This study aimed to evaluate and compare the immuno expression of glucose transporter-1 (GLUT-1) and Carbonic anhydrase IX (CA IX) between major and minor salivary pleomorphic adenomas (PAs),to better understand the differences in the biological behavior of these tumorsand determine whether these proteins participate in the development of these tumors. Thirty three cases of PAs, 16 major and 17 minor were submitted to morphological and immunohisto chemical analysis. Immuno expression of GLUT-1 and CA IX was evaluated semi quantitative fields in all cases. The cases of GLUT 1 were classified according to the scores: 0 (0% positive cells), 1 (<25% of positive cells), 2 (25–50 % of positive cells), and 3 (>50% of positive cells) While for CA IX cases were classified according to the scores: 0 (0% positive cells), 1 (<10% of positive cells), 2 (10–50 % of positive cells), and 3 (>50% of positive cells. The statistical analysis was performed using Pearson's chi-square, Fisher's exact test. Higher GLUT-1 and CAIX immuno expression was observed in the minor PA compared to major PAs (p = 0.05, 0.01) respectively. Results suggest that differences in biological behavior of the studied tumors are related to GLUT-1 Which may be involved in the metabolic regulation of glucose in PAs. In addition, CA IX appears to influence the development of major and minor PA which can explain the differences their biological behavior.