Effect of Aqueous Extract of Nigella sativa and Trigonella Foehum on Entamoeba histolytica

Background: Entamoeba histolytica is a protozoan parasite that is considered as the causative agent of amoebiasis and is considered a leading parasitic cause of human mortality. Nigella sativa (black seed) is an annual herbal plant belonging to the family . Ranundaceae. Trigonella foenum is an annual herb from the family fabaceae. The aim of current study was to study the efficiency of the aqueous extracts of nigella sativa and trigonella foenum as alternative treatments for E. histolytica infection.

Method: From October 2017 to December 2017 collected 100 feces sample in Ibn Al-balady hospital and Al-Kadimiyah teaching hospital in Baghdad from patients in hospital. their age ranged from (4-32) yea. This study was conducted to determine the effect of aqueous extracts of Nigella sativa and Trigonella fornum plant on 120 (80 mice) treated with N. sativa and T. foenum (20) mice positive control and 20 mince negative control. Swedish mice aged (5-12) weeks and the range of their weight (16-22), infected with parasite of Entamoeh histolytica experimentally and then knwong some his to pathological (in vivo) changes in those mice after using the extracts as a treatment for those mice from E-histolytice and then compare it with metronidazole.

Results: data from current study showed diverse histopathological changes in the intestines, liver, kidney in animals infected with E-histolytice and treated by N-sativa and T-foenuu extract included shortness in intestinal villi, inflammntory cellular infiltration, hyperplasia, necrosis, bloody vessel congestion and accumulation of fats. The best results were recorded in group that treated with flagyl/aqueous N, sativa 10mg/ml. In vitro, the aqueous extract of Niqella sativa killed 280.000 amoeba or %70, but 10mg/ml extract killed 320.000 ameoba by 70%. The aqueous extract of trigonella foenm (5mg/ml) killed 240.000 amoeba by 70%. However, mestronidazole killed 360.000 amorba by 90%